Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3709

Full Length Research Paper

Inhibitory effects of Anadenanthera colubrina (Vell.) Brenan stem bark extract on α-glucosidase activity and oxidative stress

Odelio Joaquim da Costa
  • Odelio Joaquim da Costa
  • Laboratório de Pesquisas em Produtos Naturais, Universidade Federal do Tocantins, City Palmas, State of Tocantins, Postcode 77020-210, Brazil.
  • Google Scholar
Robson dos Santos Barbosa
  • Robson dos Santos Barbosa
  • Laboratório de Pesquisas em Produtos Naturais, Universidade Federal do Tocantins, City Palmas, State of Tocantins, Postcode 77020-210, Brazil.
  • Google Scholar
Ilsamar Mendes Soares
  • Ilsamar Mendes Soares
  • Instituto Federal de Educação Ciência e Tecnolofia do Tocantins, City Araguatins, State of Tocantins Postcode 77.950-000, Brazil.
  • Google Scholar
Eber Euripedes de Souza
  • Eber Euripedes de Souza
  • Laboratório de Pesquisas em Produtos Naturais, Universidade Federal do Tocantins, City Palmas, State of Tocantins, Postcode 77020-210, Brazil.
  • Google Scholar
Luís Fernando A. Gellen
  • Luís Fernando A. Gellen
  • Laboratório de Pesquisas em Produtos Naturais, Universidade Federal do Tocantins, City Palmas, State of Tocantins, Postcode 77020-210, Brazil.
  • Google Scholar
João Pedro Pereira P. Lemos
  • João Pedro Pereira P. Lemos
  • Laboratório de Pesquisas em Produtos Naturais, Universidade Federal do Tocantins, City Palmas, State of Tocantins, Postcode 77020-210, Brazil.
  • Google Scholar
Raimundo Wagner S. Aguiar
  • Raimundo Wagner S. Aguiar
  • Departamento de Biotecnologia, Universidade Federal de Tocantins, City Gurupi, State of Tocantins, Postcode 77413-070, Brazil.
  • Google Scholar
Adão Lincon B. Montel
  • Adão Lincon B. Montel
  • Laboratório de Pesquisas em Produtos Naturais, Universidade Federal do Tocantins, City Palmas, State of Tocantins, Postcode 77020-210, Brazil.
  • Google Scholar
Sérgio Donizeti Ascêncio
  • Sérgio Donizeti Ascêncio
  • Faculdade de Medicina, Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Amazônia Legal - Bionorte, Laboratório de Pesquisas em Produtos Naturais, Universidade Federal do Tocantins, City Palmas, State of Tocantins, Postcode 77020-210, Brazil.
  • Google Scholar


  •  Received: 10 August 2020
  •  Accepted: 05 October 2020
  •  Published: 30 November 2020

Abstract

In this study, we investigated the promising potential of Anadenanthera colubrina bark extract (BEAc) as a product to combat diabetes mellitus (DM). We evaluated the inhibitory effects of BEAc on α-glucosidase and the oxidation of biomolecules, as well as its main phytoconstituents. In terms of free radical scavenging, BEAc exhibited a dose-effect relationship. BEAc was more efficient than rutin and butylated hydroxytoluene and similar to ascorbic acid at the same concentrations. Evaluation of the IC50 confirmed the good activity of BEAc compared to positive controls and statistically determined to be equal to ascorbic acid. In in vitro α-glucosidase inhibition studies, BEAc generated 31 times more potent inhibition than acarbose and was dose-dependent at the concentrations tested. Lineweaver-Burk and Michaelis-Menten plots obtained for kinetic analysis showed that BEAc competitively inhibited the α-glucosidase catalyzed reaction. Chemical analysis of BEAc by HPLC revealed that the plant is rich in phenolic compounds and confirmed its capacity to inhibit α-glucosidase. Fourteen compounds were identified by reference to authentic standards: Gallic acid, catechin, syringic acid, chlorogenic acid, p-coumaric acid, naringin, vitexin, rutin, isorhamnetin, hesperidin, myricetin, morin, rosmarinic acid, and quercetin. Thus, this study provides the first evidence of the antidiabetic activity of A. colubrina bark and determines its possible modes of action on carbohydrate metabolism via inhibition of α-glucosidase and the control of biomolecule oxidation. These data support the potential use of this plant for the development of promising multi-target therapy products combining postprandial hyperglycemia control and biomolecule oxidation control.

Key words: Kinetic analysis, phenolic content, diabetes mellitus, oxidation of biomolecules.