Abstract

To explore the differences of rhizo-bacterial diversity among different habits and the relationship between the quality of Fritillaria thunbergii and microbial diversity, the soil bacterial diversity was analyzed by denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR) and the content of peimine and peiminine in F. thunbergii was detected by high performance liquid chromatographic method with evaporative light scattering detection (HPLC-ELSD). For the DGGE analysis, the genetic diversity based on Shannon index of rhizo-bacterial community was the highest in Nantong (NT), followed by Chunan (CA), Panan (PA) and Ningbo (NB). Furthermore, it was significantly higher in rhizosphere soil than in non-rhizosphere soil, which indicated that some kinds of bacteria related to the growth of F. thunbergii were accumulated in rhizoshpere. Bacterial diversity based on principal component analysis (PCA) showed significant differences among all samples obtained from different habits and are divided into three major taxa: NB, CA and PA. It was found that the highest content of peimine (0.1364%) and peiminine (0.0518%) in NT, while the lowest peimine in NB (0.0646%) and the lowest peiminine (0.0316%) in PA. Moreover, the rhizo-bacterial diversity was relatively high related to the alkaloid concentration of F. thunbergii.

Key words: Traditional Chinese medicine, Fritillaria thunbergii, 16S r DNA, PCR-DGGE, microbial diversity, HPLC-ELSD, alkaloid.