Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

High frequency regeneration and shoot multiplication in Andrographis lineata wall. ex. nees: an endemic medicinal plant of south India

V. Sudarshana Deepa, K. Rajaram, M. Anis Kumar, Soni Das and P. Suresh Kumar*
Department of Biotechnology, Anna University of Technology, Tiruchirappalli-620024, Tamilnadu, India.
Email: [email protected]

  •  Accepted: 26 August 2011
  •  Published: 30 September 2011

Abstract

An attempt was made to micropropagate the potential medicinal plant Andrographis lineataWall. ex. Nees using shoot tip and nodal explants. Maximum number of shoots were produced from shoot tip explants (25.7 ± 0.11 shoots /explants) in the optimized concentration of the MS medium containing 1.5 mg/l BAP in combination with 30.0 mg/l AdS compared to the nodal explants (15.0 ± 0.19 shoots/explants). The juvenile in vitroregenerated shoots were elongated (7.0 ± 0.6 cm) in 0.3 mg/l GA3. Rooting was best induced in MS medium supplemented with 1.0 mg/l IBA. The plantlets were successfully hardened in the plant growth chamber and later transferred to greenhouse at 70% survival rate.

 

Key words: Andrographis lineata, shoot tip explants, nodal explants.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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