September 2011
Establishment of a practical tissue culture for producing hairy roots of Valeriana officinalis L. via Agrobacterium rhizogenes
Valerian (Valeriana officinalis L.) is used in traditional medicine for its root or rhizome as a mild sedative and tranquilizer in many countries. In order to evaluate the potential production of hairy root of V. officinalis under in vitro conditions the regeneration of V. officinalis via indirect organogenesis and also Agrobacterium-mediated transformation was conducted. Leaves, stems...
September 2011
Composition, antimicrobial and antioxidant activities of the essential oil of Artemisia kermanensis Podl., an endemic species from Iran
The essential oil of the aerial parts of Artemisia kermanensis was analyzed by GC-FID and GC-MS methods. Twenty-five compounds, accounting for 92.3% of the total oil, were identified. The main components of the oil were isoborneol (21.5%) and camphor (9.8%). The oil was found to be rich in regards to oxygenated monoterpenes (80.2%). The antimicrobial activity of oil was...
September 2011
In vitro conservation and low cost micropropagation of Cochlospermum regium (Mart. Ex. Scharank)
Cochlospermum regium (Mart. Ex. Scharank), a Cochlospermaceae is a Cerrado medicinal species showing antimicrobial activity on the female reproductive system. The species risks being genetically eroded since it is highly explored, the biome devastated and the plant roots are employed for phytotherapeutic preparations. This study aims to develop a micropropagation protocol for large scale production of plantlets and...
September 2011
Evaluation of the antioxidant activity of phenolic compounds among blueberry cultivars by HPLC-ESI/MS and on-line HPLC-ABTS system
Antioxidant compounds are now of considerable interest due to their effect of preventing or delaying aging and their apparent involvement in prevention of common cancers. In the present study, the fruits and leaves from a number of high bush blueberry cultivars (Vaccinium corymbosum L.) were investigated for their antioxidant capacity. The antioxidant constituents in the blueberry fruit were identified by LC-ESI/MS...
September 2011
The effects of sage volatile oil (Salvia officinalis) and Turkish oregano volatile oil (Origanum onites) on stored cowpea (Vigna nisensis L.) seed
Physical and biological quality of seed is a very important activity when cowpea seed have to be stored for long time. The treatment of chemicals inhibitors in stores is a common practice to prevent the quality of seed. The effects of different concentrations (0, 250, 500, 1000 and 2000 ppm) sage (Salvia officinalis) and Turkish oregano (Origanum onites) on cowpea seeds in stroge. The study was arranged in...
September 2011
Antimutagenic potential of chickrassy (Chukrasia tabularis A. Juss.) bark
The present study aims to assess the antimutagenic potential of methanol extract and different fractions (hexane, ethyl acetate and butanol) of chickrassy (Chukrasia tabularis), belonging to family meliaceae by employing histidine point reversion assay. The antimutagenic effect was evaluated against mutagens, 4-Nitro-o-phenylenediamine and sodium azide and promutagen, 2-Aminofluorene of TA98 and TA100 strain...
September 2011
Application of Carum copticum and Satureja hortensis essential oils and salicylic acid and silver thiosulfate in increasing the vase life of cut rose flower
The aim of this study was to investigate the potential application of Carum copticum andSatureja hortensis essential oils and salicylic acid (SA) and silver thiosulfate (STS) for preservation postharvest quality sensors of rose cut flowers. In this study, cut rose flowers were treated with alone application of this substances and their combination. Results showed that the pH of vase solution in cut...
September 2011
Improvement of the vase life of cut gladiolus flowers by essential oils, salicylic acid and silver thiosulfate
Effects of the application of salicylic acid (SA) (1.5 mMol), ajowan and summer savory essential oils (500 and 1000 ppm), silver thiosulphate (STS) (150 ppm) and combinations of these materials on vase life of cut gladiolus flowers were investigated. Results of this study showed that within all treatments, SA treatment showed the best effect on the fresh weight (%), water uptake (cm3) and vase life of cut flowers....
September 2011
High frequency regeneration and shoot multiplication in Andrographis lineata wall. ex. nees: an endemic medicinal plant of south India
An attempt was made to micropropagate the potential medicinal plant Andrographis lineataWall. ex. Nees using shoot tip and nodal explants. Maximum number of shoots were produced from shoot tip explants (25.7 ± 0.11 shoots /explants) in the optimized concentration of the MS medium containing 1.5 mg/l BAP in combination with 30.0 mg/l AdS compared to the nodal explants (15.0 ± 0.19...
September 2011
Effects of extraction techniques on phenolic components and antioxidant activity of Mengkudu (Morinda citrifolia L.) leaf extracts
The effect of four extraction techniques namely; solvent extraction (SE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE) and supercritical fluid extraction (SFE) on the phenolic components and antioxidant activity of Morinda citrifolia leaf extracts was investigated. Total phenolics compounds (TPC) were quantified spectrophotometrically using Follin-Ciocalteu regent method,...
September 2011
In vitro antibacterial and antifungal activity of Iraqi propolis
The study was aimed at determining the antimicrobial activities of crude ethanolic extract of Al-Museiab propolis (EEMP) against some bacterial and fungal isolates by the method of disc diffusion and agar-well diffusion, respectively. MICs of propolis extracts using the two-fold agar dilution susceptibility method were also determined. Results revealed thatStaphylococcus aureus was highly sensitive to EEMP than...
September 2011
Palynomorphological characterization of some species of Ficus L. from Pakistan
The present study was confined to pollen morphology and pollen fertility estimation using a taxonomic description of 11 species of genus Ficus family Moraceae. The species wereFicus bengalensis L, Ficus microcarpa L.f., Ficus palmata Forrsk., Ficus religiosa L., Ficus lacor Ham., Ficus pumila L., Ficus hispida L.f., Ficus...
September 2011
Antioxidant activity of aqueous extracts of different caffeine products
The antioxidant activity of water extracts (cold and hot) of six caffeine products were carried out. The extracts were screened for total polyphenol contents and antioxidant activity using DPPH, ABTS methods and reducing power method at 50 and 100 µg/ml after 15 and 30 min using BHA and Caffeine as standard compounds. The results indicated that, the hot water extracts for different caffeine products showed high...
September 2011
Variability of two essential oils of Ammi visnaga (L.) Lam. a traditional Tunisian medicinal plant
This study deals with the valorization of medicinal and aromatic plants of the Tunisian flora, in order to find new bioactive natural products. The essential oil constituents from the fruits ofAmmi visnaga, collected from two Tunisian localities, Ichkeul and Djebba the North of Tunisia, were extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). Forty-one compounds were...
September 2011
Studies on the effect of pomegranate (Punica granatum) juice and peel on liver and kidney in adult male rats
The pomegranate has been traditionally used as medicines in many countries. The study aimed to investigate the antioxidant properties of pomegranate in hepatic and renal tissues of rats. Eighteen adult male albino rats were randomly divided into three groups, six rats of each. The first group served as control and received saline (0.2 ml saline/ rat) by oral administration via epigastric tube. The second group...
September 2011
Antifungal activity of the essential oil of Iranian medicinal plants
Plant materials continue to play a major role in primary health care as therapeutic remedies in many developing countries. Medicinal plants contain physiologically active principles that over the years have been exploited in traditional medicine for the treatment of various ailments as they contain anti-microbial properties. Antifungal activities of essential oil of four Iranian herbs...
September 2011
Garlic-mode treatment effects on rat brain redox status
Garlic (Allium sativum) has played an important medicinal role in human history. We analyzed the ability of high dosage garlic administered per orally (PO) or via intraperitoneal (IP) route to act on brain antioxidant status in rats. In this organ, PO garlic is antioxidant as it decreased malondialdehyde (MDA), hydrogen peroxide (H2O2) and lactate dehydrogenase (LDH) levels. As a confirmation, oral garlic...
September 2011
Cytotoxic flavonoids from Diplotaxis harra (Forssk.) Boiss. growing in Sinai
As part of our ongoing collaborative effort to discover the anticancer activity of the phenolics isolated from terrestrial plant sources, the EtOH extract of the aerial parts of the Egyptian medicinal plant Diplotaxis harra (Forssk.) Boiss. was in vitro investigated for cytotoxicity against HCT116, HepG2 and MCF-7 cell lines, and resulted with IC50 = 4.65, 12.60 and 17.90 µg/ml,...
September 2011
Allelopathic potential of para-hydroxybenzoic acid and coumarin on canola: Talaieh cultivar
Phenolic compounds are secondary metabolites that can affect plant growth and development. In the present study biochemical and physiological responses of canola, Talaieh cultivar, against two allelochemical substance (para-hydroxybenzoic acid and coumarin) was investigated. In this research, chlorophyll, carotenoid and anthocyanin content were determined spectrophotometrically, while guaicol peroxidase and catalase...
September 2011
Effects of plant density and sowing date on the growth, flowering and quantity of essential oil of Calendula officinalis L.
Pot marigold (Calendula officinalis L.) is a medicinal and ornamental plant. In an experiment which was designed in factorial on the basis of completely randomized block design with 3 replicates in 36 plots. The effects of plant density (20, 40, 60 and 80 plants/m2- factor A) and sowing date (April 19 and 29, and May 10, 2010- factor B) were evaluated on the growth, flowering and essential oil of C....
September 2011
In-vitro evaluation of the antimicrobial activity of extracts of Bridelia micrantha on selected bacterial pathogens
Medicinal plants have been age long remedies for human diseases because they contain components of therapeutic value. In this study, six solvent extracts [dichloromethane, ethyl acetate, acetone, ethanol, methanol (100%), and 40% methanol hydroxide] of the stem bark of Bridelia micrantha and ciprofloxacin were investigated for antimicrobial activity by the agar-well diffusion method against strains...
September 2011
Acute toxicity studies of the aqueous root extract of Lawsonia inermis Linn. in rats
The study evaluated the lethal dose LD 50 of the aqueous root extract of Lawsonia inermis; a potent uterine vaso constrictor, usually taken by pregnant women in the first two months of pregnancy to induce spontaneous abortion. The study was carried out to determine the lethal dose of the aqueous root extract to ascertain its safety using Wister rats. Five groups of rats (4 rats per group; including two...
September 2011
Chemical constituents, antimicrobial and antioxidant activity of the hexane extract from root and seed of Levisticum persicum Freyn and Bornm.
The hexane extracts from root and seed of Levisticum persicum Freyn and Bornm, which were collected from northwestern Iran were obtained by Soxhlet apparatus. The fatty acids were derived to methyl esters and determined by gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS) systems. The extracts from the root and seed were characterized by a high amount...
September 2011
A unique water soluble formulation of β-asarone from sweet flag (Acorus calamus L.) and its in vitro activity against some fungal plant pathogens
An antifungal substance β-asarone was isolated from ethyl acetate extract of Acorus calamus L. As β-asarone is lipophilic, it was made into water soluble formulation by encapsulating into beta methyl cyclodextrin (BMCD). At β-asarone concentration of 30 ppm, this formulation completely inhibited mycelial growth of major plant pathogenic fungi,Phytophthora capsici, which was isolated from...
September 2011
Nematicidal potential of Impatiens bicolor Royle
This study was designed to evaluate the nematicidal effects of Impatiens bicolor Royle extracted with various solvent systems to explore its potential use in agriculture as pesticide. Extracts of I. bicolor Royle obtained from n-hexane (A), dichloromethane (B), ethyl acetate (C), n-butanol (D), aqueous (E) fractions as well as crude (F) were tested in vitro for their nematicidal...
September 2011
Cytotoxic effect of Mentha spicata aqueous extract on cancerous cell lines in vitro
Mentha spicata is a herb with several biological properties. Cytotoxicity of essential oils ofM. spicata on some cancer cells has been reported. In this study the cytotoxicity of aqueous extract of M. spicata on two tumor cell lines (Wehi-164 fibrosarcoma and U937 leukemic monocyte) has been evaluated in vitro. Wehi-164 and U937 cells were separately cultured in RPMI with 10% FBS. Then the...
September 2011
Application times and concentration of humic acid impact on aboveground biomass and oil production of hyssop (Hyssopus ofï¬cinalis)
The objective of this study was to find the influence of different humic acid concentration and foliar application times on aboveground biomass and essential oil production of hyssop (Hyssopus ofï¬cinalis L.) in 2008 and 2009 at experimental field of Ferdowsi University of Mashhad in Iran. The field experiment was a factorial based on randomized complete block design with three replications. Two times of foliar...
September 2011
The study of antifungal effects of herbal essences on Aspergillus parasiticus, an aflatoxin producer in pistachio (Pistacia vera)
Antifungal effects of 6 herbal essences (thyme, oregano, spearmint, lime, eucalyptus and galbanum) that seemed to have powerful antifungal characteristics on Aspergillus parasiticus were studied in this research. A. parasiticus is one of the most important species that produces aflatoxin in pistachios. The herbal essences of the used concentrations had no effects on the flavor of...
September 2011
Nutraceutical potential and bioassay of Apium graveolens L. grown in Khyber Pakhtunkhwa-Pakistan
The present study investigates the nutritive and pharmacological potential of Apium graveolens commonly available as wild plant in Peshawar and suburbs of Khyber Pakhtunkhwa region of Pakistan. For this purpose, plants of A. graveolens L. was collected from its natural habitat at mature stage (seedling stage) from Palusi, near KPK Agricultural University, Peshawar Pakistan. Similarly,...
September 2011
Antibacetial screening of leaves and stem of Carica papaya
The purpose of the study was to investigate antibacterial activity of ethanolic extracts of leaves and stem of Carica papaya on selected microorganisms. Various Gram negative (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella typhi, Salmonella paratyphi A, Shigella flexenert) and Gram positive bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis,...
September 2011
Assessment of antioxidative activities of extracts from selected Plantago species
Majority of the worlds population is still relying on natural products, infact synthetic drugs have revolutionized the modern pharmaceuticlas. Plantago have wide range of bioactive compounds. This study involved antioxidative activities of extracts from three Plantago species (Plantago ovata, Plantago lanceolata and Plantago maxicana) by ascorbate assay and catalase assay. All extracts...
September 2011
Regulation of ethylene biosynthesis by nitric oxide and thidiazuron during postharvest of rose
Nitric oxide (NO) and thidiazuron (TDZ) have been shown to extend the postharvest life of a range of flowers possibly by downregulating ethylene production. In this study, we have evaluated the effect of sodium nitroprusside (SNP), a nitric oxide donor and thidiazuron on postharvest senescence of cut Rosa flower. Therefore, we examined the effects of SNP and TDZ on its ethylene...
September 2011
Simultaneous determination of three bioactive homoisoflavanones in rhizomes of Polygonatum odoratum
An high-performance liquid chromatographic- photodiode array detector method (HPLC-PAD) method was established to simultaneously determine three bioactive homoisoflavanones in the rhizomes of Polygonatum odoratum (Mill) Druce. The optimal conditions of extraction were achieved using reflux extraction with methanol-water (80:20, v/v) for 2 h. The analysis was performed on a Diamonsil ODS column...
September 2011
Antioxidative effect of curcumin (Curcuma longa) on lipid peroxidation and lipofuscinogenesis in submandibular gland of D-galactose-induced aging male mice
Extract of turmeric (Curcuma longa) that is, curcumin was examined for antioxidant activity. The study deals with the effects of curcumin on lipid peroxidation and fluorescence product in the control, D-galactose-induced aged, D-galactose aging protected and D-galactose aging cured mice. The level of lipid peroxidation in terms of malondialdehyde and the level of lipofuscin granules in term of fluorescence product...
September 2011
Plant species diversity along the altitudinal gradient at Garhi Dopatta Hills, Muzaffarabad
The present study reveals vegetation diversity and species richness along the altitudinal gradient ranged from 900 to 3000 m at Garhi Dopatta Hills. Species diversity and richness values were high in the tree layer in the middle part of the altitudinal gradient. It decreases both towards upper and lower altitude, which was due to deforestation, human interaction, encroachment pressure, low number of species and soil...
September 2011
Assessment of ethno-medicinal plants from Chamundi Hill, Mysore
A survey has been done in Chamundi Hill of Mysore District for investigations of ethano-medicinal plants. About 34 plants were reported in this preliminary study which was used for various diseases. This manuscript is very useful for those who working with herbal plants especially doctors and researchers and those who are practicing Indian medicine system. Key words: Chamundi Hill, ethno-medicinal,...
September 2011
Morus nigra - L. A.
September 2011
Vitamin A: A review article
September 2011
Glycogen metabolism and glycogen storage diseases: A review
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