Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3812

Full Length Research Paper

Inhibiting effects of epigallocatechin gallate (EGCG) on the formation of age pigment in vitro and in vivo

Shuxian Cai 1, Jianan Huang1, Li-li Wang1, Xin-rong Dong1, Yu-shun Gong1, Juan Li1, Qing Li1, Zhong-hua Liu1,2 * and Guo-an Luo2
1National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals and Key Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha 410128, People’s Republic, China. 2Department of Chemistry, Tsinghua University and Key Laboratory of Biological Organic, Phosphorus and Chemical Biology of Ministry of Education, Beijing 100084, People’s Republic, China.
Email: [email protected]

  •  Accepted: 22 June 2011
  •  Published: 23 October 2011


Lipofuscin age pigment is often considered as a hallmark of aging, which shows an accumulation rate inversely correlated with longevity and it is primarily composed of carbonyl-amino cross linked protein residues. The purpose of this study is to determine whether epigallocatechin gallate (EGCG) would inhibit the formation of age pigment in both MDA-modified human serum albumin and D-galactose-induced aging mice models by using spectrofluorometry, HPLC-PDA, FTIR spectrometer, SEM and TEM observation. Results showed that EGCG could inhibit the formation of age pigment in vitro and in vivo. The structure-activity relationship suggested that besides anti-oxidative stress and trapping the reactive unsaturated aldehydes, the inhibiting effects were correlated with the galloyl D-ring of tea catechins on neutralizing the amino-carbonyl cross-linking reaction.


Key words: Epigallocatechin gallate (EGCG), age pigment, galloyl group, amino-carbonyl cross-linking products.