Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Optimization of enzyme assisted extraction of polysaccharides from Poria cocos

Ashfaque A. Khaskheli
  • Ashfaque A. Khaskheli
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
  • Search for this author on:
  • Google Scholar
Shahzor G. Khaskheli
  • Shahzor G. Khaskheli
  • Institute of Food Sciences and Technology, Sindh Agriculture University, Tandojam 71000, Pakistan.
  • Search for this author on:
  • Google Scholar
Ying Liu
  • Ying Liu
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
  • Search for this author on:
  • Google Scholar
Saghir A. Sheikh
  • Saghir A. Sheikh
  • Institute of Food Sciences and Technology, Sindh Agriculture University, Tandojam 71000, Pakistan.
  • Search for this author on:
  • Google Scholar
Yan-Feng Wang
  • Yan-Feng Wang
  • Mudanjiang Sub-Academy, Heilongjiang Academy of Agricultural Sciences, Mudanjiang, Heilongjiang, 157041, China.
  • Search for this author on:
  • Google Scholar
Aijaz H. Soomro
  • Aijaz H. Soomro
  • Institute of Food Sciences and Technology, Sindh Agriculture University, Tandojam 71000, Pakistan.
  • Search for this author on:
  • Google Scholar
Xiaojiu Tian
  • Xiaojiu Tian
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
  • Search for this author on:
  • Google Scholar
Mamoun A. Homaida
  • Mamoun A. Homaida
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
  • Search for this author on:
  • Google Scholar
Wen Huang*
  • Wen Huang*
  • College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
  • Search for this author on:
  • Google Scholar


  •  Received: 25 February 2017
  •  Accepted: 06 April 2017
  •  Published: 03 May 2017

Abstract

Water-soluble polysaccharide was isolated from Poria cocus by enzyme-assisted method using orthogonal methodology. By single factor test and orthogonal group, the extraction conditions of water-soluble polysaccharide were investigated, such as liquid-solid ratio, temperature, time and pH. The optimum conditions for single factor were as follows Enzyme concentration (%), Extraction temperature oC, Extraction time/h, Extraction pH. The result revealed that 2% complex enzyme, remained most important factor of polysaccharide extraction, followed by temperature and pH. The liquid-solid ratio was 1:50, temperature was 40°C, time was 3.0 h, and pH was 5. The highest extraction rate of crude polysaccharide remained 4.14%. Results indicated that the complex-enzyme assisted remained best technique for extracting polysaccharide from P. cocus. It proved to be as highly effective as well as energy and time saving extraction techniques.

Key words: Poria cocus enzyme assisted extraction, polysaccharides, alpha amylase, cellulase, Taka-diastase.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 11 No. 17
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES