Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3843

Full Length Research Paper

Application of a degreasing process and sequential ultrasound-assisted extraction to obtain phenolic compounds and elucidate of the potential antioxidant of Siparuna guianensis Aublet

Ilsamar Mendes Soares
  • Ilsamar Mendes Soares
  • Research Laboratory of Natural Products, Biodiversity and Biotechnology Post Graduate Program of the Amazon (Bionorte), Federal University of Tocantins, Palmas, Tocantins 77020-210, Brazil.
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Maria de Fátima Ribeiro2,
  • Maria de Fátima Ribeiro2,
  • Research Laboratory of Natural Products, Agroenergy Post Graduate Program,Federal University of Tocantins, Palmas, Tocantins 77020-210, Brazil.
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Odelio Joaquim da Costa1,
  • Odelio Joaquim da Costa1,
  • Research Laboratory of Natural Products, Biodiversity and Biotechnology Post Graduate Program of the Amazon (Bionorte), Federal University of Tocantins, Palmas, Tocantins 77020-210, Brazil.
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Éber Eurípedes de Souza1
  • Éber Eurípedes de Souza1
  • Research Laboratory of Natural Products, Biodiversity and Biotechnology Post Graduate Program of the Amazon (Bionorte), Federal University of Tocantins, Palmas, Tocantins 77020-210, Brazil.
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, Aline Aires Aguiar1,
  • , Aline Aires Aguiar1,
  • Research Laboratory of Natural Products, Biodiversity and Biotechnology Post Graduate Program of the Amazon (Bionorte), Federal University of Tocantins, Palmas, Tocantins 77020-210, Brazil.
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Robson dos Santos Barbosa3
  • Robson dos Santos Barbosa3
  • Agroenergy Post Graduate Program, Federal University of Tocantins, Palmas, Tocantins 77020-210, Brazil.
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, Tarso da Costa Alvim4,
  • , Tarso da Costa Alvim4,
  • Faculty of Food Engineering, Agroenergy Post Graduate Program, Federal University of Tocantins, Av. NS 15, Block Number III, 26 Room, Palmas, Tocantins 77020-210, Brazil.
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Sérgio Donizeti Ascêncio5*
  • Sérgio Donizeti Ascêncio5*
  • Faculty of Medicine, Research Laboratory of Natural Products, Biodiversity and Biotechnology Post Graduate Program
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Raimundo Wagner de Souza Aguiar6
  • Raimundo Wagner de Souza Aguiar6
  • Department of Biotechnology, Biodiversity and Biotechnology Post Graduate Program of the Amazon (Bionorte) Gurupi, Federal University of Tocantins, Tocantins 77404-970, Brazil.
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  •  Received: 07 April 2017
  •  Accepted: 23 May 2017
  •  Published: 03 June 2017

Abstract

This study investigated appropriate means to determine the total phenolic content and antioxidant activity of the leaves of Siparuna guianensis. Evaluation by thin layer chromatography (TLC) revealed phenolic compounds in all extracts. The results were confirmed by high-performance liquid chromatography (HPLC), with the identification and quantification of catechin, epigallocatechin gallate and rutin. The methanolic extract had the highest content of phenolic compounds, total tannins and condensed tannins (221.16 ± 3.39 mg GAE/g, 183.72 ± 1.85 mg GAE/g and 135.92 ± 1.00 μg/mL CE/g, respectively) and the strongest radical scavenging activity (IC50 = 37.54 ± 0.41 and 73.13 ± 2.09 μg/mL for DPPH and ABTS, respectively). The ethanolic and hydroethanolic extracts had the highest flavonoid (111.28 ± 4.59 and 103.27 ± 3.00 mg RE/g, respectively) and flavonol content (99.73 ± 0.64 and 77.31 ± 0.33 mg RE/g, respectively). In the correlation analysis, it was verified that tannins are the main phenolic compounds and antioxidants of S. guianensis leaves, and their flavonoid content consists mainly of flavonols. The data obtained establish S. guianensis as a new alternative source of antioxidant compounds, implying its potential application as a food preservative or in the production of nutraceutical and pharmacological products.

 

Key words: Flavonoid, polyphenol, condensed tannins, flavonol, plant extract, free radical scavenging

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