Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3823

Full Length Research Paper

Anti-inflammatory activity of hot water extract of Berberis koreana in lipopolysaccharide-induced macrophage-like cells

Byung Hun Kim1, Jaehwi Lee2, Ting Shen1, Jong Dai Kim1 and Jae Youl Cho1*     
1School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.  2College of Pharmacy, Chung-Ang University, Seoul 156 -756, Korea. 
Email: [email protected]

  •  Accepted: 22 April 2010
  •  Published: 04 May 2010


Berberis koreana has been known as enthopharmacologically valuable plant in Korea, China and Japan. This plant has been reported to display numerous pharmacological activities such as anti-oxidative, neuroprotective, and anti-cancer effects. Although the pharmacological potentials have been demonstrated, anti-inflammatory effect of this plant has not been fully elucidated yet. To evaluate its anti-inflammatory activity, macrophages activated by lipopolysaccharide (LPS) were employed and the production of inflammatory mediators was explored in terms of understanding its molecular inhibitory mechanism. Hot water extract from B. koreana (Bk-HWE) was able to suppress the production of NO and TNF- production and up-regulation of surface levels of costimulatory molecules such as CD80 and CD86. The anti-inflammatory effect of Bk-HWE seemed to be due to the inhibition of MAPK activation and c-fos translocation, according to immunoblotting analysis. In addition, Bk-HWE strongly suppressed the cell-cell adhesion events induced by functional activation of adhesion molecules such as CD29 and CD43. Therefore, our results suggest that Bk-HWE can be applied as an anti-inflammatory herbal medicine. To prove this assumption, further in vivo efficacy test will be continued in the following project. 
Key words: Sorbus commixta, macrophages, inflammatory mediators, AP-1 translocation, JNK activation.