Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Immunomodulating properties of protein fractions isolated from Delphinium staphysagria seeds

Amal Bousfiha
  • Amal Bousfiha
  • Laboratory of Bioactive Molecules (LMBSF), Faculty of Sciences and Techniques, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
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Najlae Mejrhit
  • Najlae Mejrhit
  • Laboratory of Bioactive Molecules (LMBSF), Faculty of Sciences and Techniques, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
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Ouarda Azdad
  • Ouarda Azdad
  • Laboratory of Bioactive Molecules (LMBSF), Faculty of Sciences and Techniques, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
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Mohamed El Kabbaoui
  • Mohamed El Kabbaoui
  • Laboratory of Bioactive Molecules (LMBSF), Faculty of Sciences and Techniques, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
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Alae Chda
  • Alae Chda
  • Laboratory of Bioactive Molecules (LMBSF), Faculty of Sciences and Techniques, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
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Abdelali Tazi
  • Abdelali Tazi
  • Laboratory of Bioactive Molecules (LMBSF), Faculty of Sciences and Techniques, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
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Rachid Bencheikh
  • Rachid Bencheikh
  • Laboratory of Bioactive Molecules (LMBSF), Faculty of Sciences and Techniques, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
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Lotfi Aarab
  • Lotfi Aarab
  • Laboratory of Bioactive Molecules (LMBSF), Faculty of Sciences and Techniques, Sidi Mohamed Ben Abdellah University, Fez, Morocco.
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  •  Received: 22 September 2015
  •  Accepted: 30 December 2015
  •  Published: 17 January 2016

Abstract

The aim of this study was to investigate the immunomodulatory activities of protein fractions isolated from Delphinium staphysagria seeds used in Moroccan traditional medicine. D. staphysagria protein extract has been separated on sephadex column chromatography. The immunomodulatory activities were tested using primary culture of rabbit lymphocytes (splenocytes and thymocytes), by evaluation of macrophage phagocytosis and by studying the hemagglutination effect of proteins separated by chromatography. Three protein fractions were isolated on sephadex column. At 0.1mg/ml, these fractions didn’t show any cytotoxic effect against lymphocytes. The fraction F1 with the high molecular weight of 115 kDa exhibited a mitogenic action on splenocytes and positive hemagglutinating activity associated to inhibition of macrophage phagocytosis capacity. The second protein fraction F2 was about 11 kDa inhibited phagocytosis without affecting lymphocytes proliferation. The third fraction F3 was the smallest with 2.25 kDa indicated a stimulatory effect on both splenocytes and thymocytes proliferation without hemagglutinating activity but inducing decrease of macrophage phagocytosis. In conclusion, protein fractions isolated from D. staphysagria have no cytotoxic effect. The fraction F3 acted as an immunostimulating by enhancing proliferation of thymocytes and splenocytes while the F1 stimulated preferentially splenocytes via probably lectin pathway. All fractions studied decreased phagocytosis impairing the antigen presentation to lymphocytes.

Key words: Delphinium staphysagria, protein fractions, immunomodulation, mitogenic effect, hemagglutination, MTT assay.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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