Abstract

Curcuma mangga is one of the important and valuable medicinal crops of the family Zingiberaceae. This species also has a high potential for essential oil. An efficient protocol for the micropropagation of C. mangga followed by successful acclimatization to soil was developed. The aseptic rhizome buds were cultured onto Murashige and Skoog (MS) medium supplemented with different concentrations of BAP, IAA and NAA for 10 weeks. MS medium supplemented with 9 mg/L BAP was found to be the optimum for shoot multiplication while MS medium supplemented with 1 mg/L NAA gave the highest root number. The explants were then subcultured on to MS medium supplemented with different concentrations of BAP and NAA for 4 weeks. The MS medium supplemented with a combination of 3 mg/L BAP and 1 mg/L NAA gave the highest number of shoot multiplication. The plantlets were successfully acclimatized with 75% survival rate.

Key words: Curcuma mangga, micropropagation, explants, acclimatization, Zingiberaceae.

Abbreviation

MS, Murashige and Skoog; BAP, 6-benzalaminopurine; NAA,naphthaleneacetic acid; IAA, indole acetic acid.