Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

A comparative evaluation of the antioxidant activity of some medicinal plants popularly used in Nepal

Bimal Kumar Ghimire1, Eun Soo Seong2, Eun Hye Kim1, Amal Kumar Ghimeray3, Chang Yeon Yu2, Bal Krishna Ghimire2 and Ill Min Chung1*
1Department of Applied Life Science, Konkuk University, Seoul 143-701, South Korea. 2Bioherb Research Institute, Kangwon National University, Chuncheon 200-701, South Korea. 3Department of Bio-Health Technology, College of Bio-medical Science, Kangwon National University, Chuncheon 200-701, Korea.
Email: [email protected]

  •  Accepted: 01 September 2010
  •  Published: 18 May 2011


Recently, attention has focused on phytochemicals as new sources of natural antioxidants.Therefore, the methanolic crude extracts of 24 commonly used medicinal plants fromJhapa and Illam District, Nepal, were screened for total phenols, flavonoids, and free radical scavenging activity. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picryl-hydrazyl (DPPH). Significant differences in DPPH scavenging activity were found between the species investigated, ranging from 14.67 ± 1.00% to 92.33 ± 1.53%. The highest radical scavenging activity was observed in Artemisia vulgaris (92.33 ± 1.53% inhibition), followed by Ficus lacor (92.00 ± 1.73%) and Mallotus philippensis (91.33 ± 1.53%). The total phenol content of the investigated species ranged from 28.87 ± 2.36 to 321.23 ± 1.06 mg GAE/g extract, while flavonoid content ranged from 13.53 ± 0.85 to 100.33 ± 1.53 mg QE/g extract. A weak linear correlation between total phenolic or flavonoid content and antioxidant activity was found (correlation coefficient, R2 = 0.3004, R= 0.4294, respectively), indicating that the major antioxidant components might not be phenolics. In particular, A. vulgaris, F. lacorM. philippensisTrachyspermum ammi, and Amomum subulatum showed strong activity against DPPH, and thus could be used as natural antioxidants in the food and/or pharmaceutical industry.


Key words: Phenolic content, flavonoids, medicinal plants, antioxidant activity, methanolic extraction.