Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

The antibacterial activity of Azadarichta indica (neem) seeds extracts against bacterial pathogens associated with eye and ear infections

A. M. El-Mahmood1*, O. B. Ogbonna1 and M. Raji2
1Department of Microbiology, School of Pure and Applied Sciences, Federal University of Technology, P. M. B. 2086, Yola, Adamawa State, Nigeria. 2Department of Biochemistry, School of Pure and Applied Sciences, Federal University of Technology, Yola, Adamawa State, Nigeria.
Email: [email protected], [email protected]

  •  Accepted: 11 November 2009
  •  Published: 30 September 2013


The effects of crude extracts of seeds of Azadarichta indica  against pathogenic Staphylococcus aureus, Staphylococcus pyogenes, Escherichia coli andPseudomonas aeruginosa obtained as clinical isolates from patients diagnosed with eye and ear infections were investigated using the agar well diffusion method . The growth of all the isolates were inhibited, though to varying degrees, with gram-positive more susceptible than gram-negative bacteria. The control laboratory strains were more sensitive to the toxic effects of the crude extracts than the corresponding test bacteria. Hexane extracts were more effective, producing larger zones of growth inhibition sizes and smaller MIC and MBC values, than the aqueous extracts. The MIC values ranged from 1.59 -25 mg/ml while the MBC values ranged from 3.17-50 mg/ml. The extracts were more effective under elevated temperature and acidic conditions. The ability of the crude extracts to inhibit the growth of such pathogenic bacteria that frequently cause eye and ear infections as those used in this study is an indication that the neem seed has the potential and can be used as a source for new broad spectrum oral antibiotics. The result obtained in this study validates the use of the neem seeds in traditional medicine to treat infectious conditions especially those involving the eye and ear.


Key words: Azadarichta indica, neem seeds, pathogenic, disc diffusion, validates, infectious condition.