Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3830

Full Length Research Paper

The inhibitory effect of Opuntia humifusa Raf. ethyl acetate extract on platelet aggregation

Won-Jun Oh1, Mehari Endale1, Ji-Young Park1, Yi-Seong Kwak2, Suk Kim3, Gon-Sup Kim3* and Man Hee Rhee1 *
1College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea. 2Korea Ginseng Corporation Central Research Institute, Daejeon 305-805, Republic of Korea. 3College of Veterinary Medicine, Gyeongsang National University, Jinju 302-305, Republic of Korea.
Email: [email protected], [email protected]

  •  Accepted: 01 November 2010
  •  Published: 18 April 2011

Abstract

This study was designed to investigate the activity of ethyl acetate extract from Opuntia humifusa Raf. (OH-EAE) in ligand-activated platelet aggregation. Platelet aggregation was induced either by ADP, a potent agonist to platelet G protein-coupled P2Y receptor, by collagen, a potent ligand that activates platelet integrin α2β1 and glycoprotein VI, or thrombin, a platelet protease-activated receptors subtype I and IV. The OH-EAE inhibited platelet aggregation induced by ADP (10 μM) in a dose dependent manner. In addition, OH-EAE significantly and dose-dependently inhibited collagen (2.5 μg/ml)- and thrombin (0.05 U/ml)-induced platelet aggregation. Moreover, the downstream signaling analysis revealed that the extract potently inhibited ADP-induced intracellular calcium mobilization ([Ca2+li). Since degranulation is a marker of platelet activation, the extract effect on the dense granule secretary activity was evaluated. As such, OH-EAE strongly suppressed ADP-induced ATP release. This preliminary result suggests that O. humifusa may be taken as a candidate lead natural compound to be considered in the search for natural products with beneficial effects on aberrant platelet activation mediated cardiovascular disorders.

 

Key words: Opuntia humifusa extract, calcium, rat platelets, platelet agonists.

Abbreviation

OH-EAE, Ethyl acetate extract from Opuntia humifusa Raf.; GP VI,glycoprotein VI; PLCβ, Phospholipase Cβ; DAG, diacylglycerol; PKC, protein kinase C;PAR, Protease-activated receptor; ATP, Adenosine triphosphate.