Abstract

Callus and cell suspension cultures of Agastache rugosa were established to investigate the production of rosmarinic acid in vitro. For callus induction 2,4-D treatment was more effective than that of NAA. The best callus induction rate (92%) and callus growth A. rugosa were obtained in MS medium containing 2 mg/l 2, 4-D. In cell suspension culture of A. rugosa, the maximum growth (7.7 g/l) and the highest rosmarinic acid production (11.5 mg/g) were attained in the liquid B5 medium supplemented with 2 mg/l 2,4-D and 0.1 mg/l BAP at 10 days after culture. The present results demonstrate that cell culture of A. rugosa might be an alternative approach for the production of rosmarinic acid.

Key words: Agastache rugosa, callus, cell suspension, rosmarinic acid.