Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Simultaneous Ultra Performance Liquid Chromatography (UPLC) analysis of five components in Fructus aurantii-type formulae

Wei Huang1, Xi Huang1,2,3*, Zhihua Xing1, Feng Qin1, Ping Ren1, Weiping Liu1, Pingping Gan4, Xinjian Qiu1, Zhaoqian Liu5, Honghao Zhou5, Licheng Gao5 and Lan Fan5
1Laboratory of Ethnopharmacology, Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, 410008 Changsha, China. 2Key Unit of Traditional Chinese Medicine Gan of (State Administration of Traditional Chinese Medicine) SATCM, Xiangya Hospital, Central South University, 410008 Changsha, China. 3Key Unit of the 11th Five-year Plan of (State Administration of Traditional Chinese Medicine) SATCM in Cerebrosis, Xiangya Hospital, Central South University, 410008 Changsha, China. 4Oncology Department, Xiangya Hospital, Central South University, 410008 Changsha, China. 5Institute of Clinical Pharmacolgy, Central South University, 410008 Changsha, China.
Email: [email protected]

  •  Accepted: 19 November 2010
  •  Published: 04 January 2011

Abstract

A simple, rapid and sensitive liquid chromatographic method has been established for simultaneous analysis of five compounds (narirutin, hesperidin, naringin, neohesperidin and meranzin hydrate) in Fructus aurantii-type preparations. The compounds were separated in less than 12 min using a C18 column with gradient elution using (A) acetonitrile, (B) water and (C) acetic acid at a flow rate of 0.3 ml/min and with a PDA detector. The method was validated for specificity, accuracy, precision and limits of detection. Good linear regression data (r2 > 0.9980) were obtained for all the calibration plots within the ranges tested. The method is an attractive alternative for quality control and clinical monitor of F. aurantii -type preparations.

 

Key words: Fructus aurantii, formulae, chemical compounds, UPLC-PDA.

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