Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Anti-inflammatory bio-activities in water extract of Centaurea ainetensis

Rabih S. Talhouk1,3*, Wassim El-Jouni1,3,4, Riad Baalbaki2,3,5, Hala Gali-Muhtasib1,3, Joanna Kogan3 and Salma N. Talhouk2,3    
1Departments of Biology, Faculty of Arts and Sciences, American University of Beirut Riad El-Solh, Beirut 1107-2020, P. O. Box 11-0236 Lebanon. 2Crop Production and Protection, Faculty of Agriculture and Food Science,3IBSAR Center, American University of Beirut, Beirut, Lebanon. 4Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA. 5Plant Pest Diagnostics, California Department of Food and Agriculture, Sacramento, California, USA    
Email: [email protected]

  •  Published: 28 February 2007


Of a selected 29 plants endemic to Lebanon and claimed to have anti-inflammatory effects, according to Lebanese folk literature, Centaurea ainetensiswas chosen to further characterize its claimed anti-inflammatory activity using in-vitro and in-vivo assays. Water extract from C. ainetensis, at non-cytotoxic concentrations, inhibited in a dose dependant manner interleukin-6 (IL-6) and gelatinases A and B produced by endotoxin (ET)-treated CID-9 cells grown in conditions that favor differentiation, on EHS-drip, at 9, 24 and 48 h post ET treatment. The inhibition was detected in doses as low as 3% (of a 1:8 decoction) and reached maximal levels of inhibition at 8% similar to control non ET-treated cells. C. ainetensis extract at these concentrations did not affect CID-9 cell’s expression of β-casein, a milk protein expressed by functionally differentiated mammary cells in culture. In vivo studies showed that C. ainetensis extract reversed ET-induced paw edema and thermal hyperalgesia in rats as demonstrated by hot plate and heat hyperalgesia pain tests.


Key words: Inflammation, IL-6, gelatinases, mammary cells, Centaurea ainetensis.