Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Nitric oxide interacts with reactive oxygen species to regulate oligosaccharide-induced artemisinin biosynthesis in Artemisia annua hairy roots

Li Ping Zheng1, Ben Zhang2, Ting Zou2, Zhong Hua Chen3 and Jian Wen Wang2*
1Department of Horticultural Sciences, Jintanglang School of Architecture and Urban Environment, Soochow University, Suzhou 215006, P. R. China. 2School of Pharmaceutical Sciences, Soochow University, Suzhou 215006, P. R. China. 3Laboratory of Plant Physiology and Biophysics, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
Email: [email protected]

  •  Accepted: 15 April 2010
  •  Published: 04 May 2010

Abstract

This work was to characterize the generation of nitric oxide (NO) and reactive oxygen species (ROS) in Artemisia annua roots induced by an oligosaccharide elicitor (OE) from Fusarium oxysporum mycelium and their relationship with artemisinin production. In the hairy root cultures, the OE at 0.3 mg total sugar/ml evoked a rapid production of nitric oxide synthase (NOS)-dependent NO and hydrogen peroxide (H2O2). The NO donor, sodium nitroprusside (SNP) potentiated OE-induced H2O2 production. The OE-induced NOS activity was suppressed by NADPH oxidase inhibitor diphenylene iodonium (DPI) and H2O2scavenger catalase (CAT), while the induced NADPH oxidase activity was decreased by the NOS inhibitor and NO scavenger. Moreover, the OE-induced NO and ROS were involved in stimulating the artemisinin accumulation in the hairy roots. These results suggest that NO and ROS could play a role in the elicitor-induced responses and secondary metabolism activities in A. annua.

 

Key words: Artemisinin, hairy roots, nitric oxide, oligosaccharide elicitor, reactive oxygen species.