Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3758

Full Length Research Paper

Antioxidation and protection against H2O2 induced oxidative stress on normal human hepatocytes cell line (LO2) by extracts and three compounds from the root of Isatis Indigotica

Chen Han1, Cheng Yan1, Li Xiang1*, Chen Jian-Wei1, Li Jin1, Xue Ping1, Wang Sheng2and Ye Wei-Yang1
1College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China. 2JiMinKeXin ShanHe YaoYe Co. Ltd, Wuxi, China.
Email: [email protected]

  •  Accepted: 01 February 2012
  •  Published: 16 March 2012

Abstract

The effect of Isatidis indigotica Fort (banlangen) extracts with its three typical compounds on antioxidation and oxidative stress damage induced in LO2 by hydrogen peroxide has been investigated. Their antioxidant activities were assessed by two complementary test systems, namely DPPH radical scavenging activity and ferric reducing antioxidant power (FRAP) assay. Among the tested samples, considerable activity of isolariciresinol was observed in all test systems (DPPH IC50: 24.29 μM; FRAP: 7.16 mM FSE/g) compared to that of ascorbic acid as reference substance (DPPH IC50: 38.95 μM; FRAP: 0.78 mM FSE/g). The percentage of cell viability was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. This study showed that incubation with isolariciresinol, banlangen keli (BLGKL) caused significant increase in the viability of LO2. The results demonstrated that Banlangen contained hepatoprotective compounds distributed in different extracts.

 

Key words: Isatidis indigotica Fort, antioxidation, hepatocytes cell line (LO2), isolariciresinol, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT).