Abstract

The effect of Isatidis indigotica Fort (banlangen) extracts with its three typical compounds on antioxidation and oxidative stress damage induced in LO2 by hydrogen peroxide has been investigated. Their antioxidant activities were assessed by two complementary test systems, namely DPPH radical scavenging activity and ferric reducing antioxidant power (FRAP) assay. Among the tested samples, considerable activity of isolariciresinol was observed in all test systems (DPPH IC50: 24.29 μM; FRAP: 7.16 mM FSE/g) compared to that of ascorbic acid as reference substance (DPPH IC50: 38.95 μM; FRAP: 0.78 mM FSE/g). The percentage of cell viability was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. This study showed that incubation with isolariciresinol, banlangen keli (BLGKL) caused significant increase in the viability of LO2. The results demonstrated that Banlangen contained hepatoprotective compounds distributed in different extracts.

Key words: Isatidis indigotica Fort, antioxidation, hepatocytes cell line (LO2), isolariciresinol, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT).