Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Genetic diversity of the chloroplast trnL-trnF intergenic spacer and nuclear internal transcribed spacer of great burdock (Arctium lappa L.) in Taiwan

Mei-Chen Tseng1, Saou-Lien Wong1, Dai-Shion Hsiung1, Jieh-Horng Hwang2, Shih-Chiang Lee3 and Fu-An Chen2*
1Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung 912, Taiwan. 2Department of Pharmacy, Tajen University, Pingtung 907, Taiwan. 3Tong Yuan Biopharmaceutical Co. Ltd., Kaohsiang 813, Taiwan.
Email: [email protected]

  •  Accepted: 10 April 2012
  •  Published: 26 September 2012

Abstract

Great burdock (Arctium lappa L.) of the Compositae has economic importance in East Asian countries. In Taiwan, major cultivated areas are located in Gueilai Township, Pingtung County and Jiali Township, Tainan County. The present work was undertaken with two objectives of determining intraspecific genetic variations and studying the population dynamics of A. lappa L. In total, 23 individuals were collected from Gueilai (n = 15) and Jiali (n = 8). Chloroplast trnL-trnF intergenic spacer (IGS) and nuclear ribosomal DNA internal transcribed spacer (ITS) sequences were subcloned and sequenced. Length variations among IGS sequences ranged from 805 to 808 bp and among ITS sequences ranged from 360 to 362 bp. Intraspecific genetic distances within IGS sequences ranged from 0 to 0.01 and within ITS sequences ranged from 0 to 0.023, which were lower than interspecific distances. All IGS and ITS sequences being separately scattered on each of the neighbor-joining trees with insignificant genetic differentiation indices (p > 0.05) suggested that both samples shared a recent common ancestor. Minimum spanning trees, Tajima’s and Fu’s parameters, and mismatch distributions implied that A. lappa L. had experienced a historical population expansion.

 

Key words: Compositae, Gueilai, Jiali, genetic distance, population expansion.