Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3702

Full Length Research Paper

Assessment of the genotoxic and antigenotoxic potential of crude extracts and fractions of Schwartzia brasiliensis (Choisy) Bedell ex Giraldo-Cañas

Mello G. S
  • Mello G. S
  • Laboratório de Biotecnologia de Plantas, Departamento de Biologia Vegetal, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Rua São Francisco Xavier, n. 524, sala 509, 20.550-013, Rio de Janeiro, Brasil.
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De Mattos J. C. P
  • De Mattos J. C. P
  • Laboratório de Radio e Fotobiologia, Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Avenida 28 de setembro, n. 87, 4º. Andar, 20551-030, Rio de Janeiro, Brasil.
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Amaral A. C. F
  • Amaral A. C. F
  • Laboratório de Produtos Naturais 1, Instituto de Tecnologia em Fármacos - Far-Manguinhos/FIOCRUZ, Rua Sizenando Nabuco, n. 100, 21041-250, Rio de Janeiro, Brasil.
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Amorim L. M. F
  • Amorim L. M. F
  • Laboratório de Oncologia Molecular, Departamento de Biologia Celular e Molecular, Universidade Federal Fluminense (UFF), Rua Outeiro de São João Batista s/n., 24020-150, Niterói, Brasil.
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Caldeira-de-Araujo A
  • Caldeira-de-Araujo A
  • Laboratório de Radio e Fotobiologia, Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Avenida 28 de setembro, n. 87, 4º. Andar, 20551-030, Rio de Janeiro, Brasil.
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Albarello N*
  • Albarello N*
  • Laboratório de Biotecnologia de Plantas, Departamento de Biologia Vegetal, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Rua São Francisco Xavier, n. 524, sala 509, 20.550-013, Rio de Janeiro, Brasil.
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  •  Received: 15 December 2014
  •  Accepted: 12 February 2015
  •  Published: 17 February 2015

Abstract

Schwartzia brasiliensis (Marcgraviaceae) is a native Brazilian shrub species with neotropical distribution in shoal environments. Recent studies have revealed its medicinal potential for some human diseases; therefore, this study aimed to evaluate the genotoxic and antigenotoxic potential of extracts and fractions obtained from different organs of S. brasiliensis. To accomplish this, plant extracts at different concentrations (25, 125 and 250 µg/ml) were incubated with plasmid DNA, either alone, or in the presence of stannous chloride as a positive control. Samples were then examined to detect any plasmid strand breaks or the absence of such breaks, which would indicate protection of these molecules against stannous chloride-induced lesions. Methanol and aqueous extracts of leaves and stems showed the ability to withstand the effects of stannous chloride in that no DNA damage was observed. Moreover, no other extracts or fractions used in the experimental conditions assayed resulted in DNA damage. These findings suggest that S. brasiliensis has antigenotoxic properties, indicating, in turn, that its biological activities deserve further study given the medicinal relevance of this plant.

 

Key words: Medicinal plant, Marcgraviaceae, stannous chloride, flavonoids.