Abstract

The present study aims to assess the antimutagenic potential of methanol extract and different fractions (hexane, ethyl acetate and butanol) of chickrassy (Chukrasia tabularis), belonging to family meliaceae by employing histidine point reversion assay. The antimutagenic effect was evaluated against mutagens, 4-Nitro-o-phenylenediamine and sodium azide and promutagen, 2-Aminofluorene of TA98 and TA100 strain of Salmonella typhimurium. The co-incubation and pre-incubation mode of treatments were used to evaluate the bioantimutagenic and desmutagenic effects, respectively. From the results obtained, it was clear that methanol extract and its fractions showed more desmutagenic effect than bioantimutagenic effect. The methanol extract was found to be most active in TA98 while ethyl acetate fraction showed good results in TA100 strain against both promutagen and direct acting mutagen. High performance liquid chromatography (HPLC) analysis of methanol extract was carried out for the identification of chemical constituents and the results revealed that catechin, quercetin and rutin have contributed to its antimutagenic activity.

Key words: Chickrassy, bioantimutagen, desmutagen, Salmonella typhimurium, high performance liquid chromatography (HPLC).

Abbreviation

2-AF, 2-Aminofluorene; NPD, 4-nitro-o-phenylenediamine; SA, sodium azide;DMSO, dimethyl sulphoxide; HPLC, high performance liquid chromatography;

MEB, methanol extract of bark; HFB, hexane fraction of bark; EAFB, ethyl acetate fraction of bark; BFB, butanol fraction of bark; MNNG, n-methyl-n’-nitro-n-nitrosoguanidine;

4NQO, 4-nitroquinoline-n-oxide; Trp-P-1, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole acetate; B(α)P: Benzo(α)Pyrene; TFA, trifluroacetic acid;  DNA, deoxyribonucleic acid;GSTs, glutathione s-transferases; ROS, reactive oxygen species; IMTech, institute of microbial technology; DAD, diode array detector.