Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3834

Full Length Research Paper

Dan-Shen-Yin has integrated protective effects in diabetic atherosclerosis rat models

Kuipo Yan1, Yong Guo2, Zhihua Xing1*, Xing Wei1, Shengping Dai3, Xinhua Sun4 and Min Duan4
1Institute of Integrated Traditional Medicine and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, People’s Republic of China. 2Department of neurosurgery, Xiangya Hospital, Central South University, 410008 Changsha, People’s Republic of China. 3Department of cardiology, the PLA 152 central hospital, 467000, Pingdingshan, People’s Republic of China. 4Department of cardiology, the first affiliated hospital of Henan college of TCM, 450008 Zhengzhou, People’s Republic of China.
Email: E-mail:[email protected]

  •  Accepted: 22 September 2011
  •  Published: 23 November 2011


Dan-Shen-Yin (DSY) is a famous traditional Chinese formula. We determined its integrated protective effects mechanisms of DSY in diabetic atherosclerosis rat model. DSY (5 or 10 g/kg/day for 4 weeks) was orally administrated to the diabetic atherosclerosis rats, which were induced by nitric oxide inhibition ( L-NAME in drinking water, 1 mg/ml) plus high-fat diet. At the end of the experiments, the serum level of malondialdehyde (MDA), total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and GSH-peroxidase (GPX) activities and the serum level of glutathione(GSH) were measured; The concentrations of circulating inflammatory markers C-reactive protein (CRP), interleukin-6 (IL-6) and tumour necrosis factor-a (TNF-α) and serum fructosamine were determined, daily food and water intakes were measured and body weights were recorded every week. Also, we determined the total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C), and the mRNAs expression of monocyte chemoattractant protein (MCP)-1, intercellular adhesion molecule (ICAM)-1 and CD36 mRNA in aorta. The results demonstrated that DSY had no direct effect in lowering serum fructosamine, but can produce a higher survival rate less body weight loss and more water intake in diabetic atherosclerosis in GK rats,which may be through anti-oxidation, anti-inflammation, regulating blood lipid, inhibiting expression of genes related to foam cell formation in rat aorta. Dan-Shen-Yin may be used as a promising agent for both prophylaxis and treatment of diabetic atherosclerosis.


Key words: Chinese medicine formula, Dan-Shen-Yin, diabetic atherosclerosis, oxidative stress, inflammation, blood lipid, genes related to foam cell formation, diabetic GK rats.