Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Antibacterial activity and cytotoxicity of the leaf essential oil of Morus rotunbiloba Koidz

Thipamon Patharakorn1, Teerakul Arpornsuwan2, Nuanchawee Wetprasit3, Amornrat Promboon4 and Sunanta Ratanapo4*
1Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand. 2Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathumthani, Thailand. 3Department of Biotechnology, Faculty of Science, Ramkhamhaeng University, Bangkok, Thailand. 4Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Email: [email protected]

  •  Accepted: 20 April 2010
  •  Published: 04 May 2010


Essential oils from the leaf of Morus rotunbiloba Koidz was extracted and analysed using steam distillation and GC-MS. The results obtained indicated that the main components of the extracts are benzyl alcohol (10.48%),dihydroactinolide (10.11%) and palmitic acid (7.01%). The oil possessed strong antibacterial activity against Vibrio cholerae O139 DMST 9701, Samonella paratyphi A DMST 8486, Serratia marcescens ATCC 8100 and Samonella choleraesuis ATCC 10708 with minimum inhibition concentration of 1.33, 2.66, 2.66 and 10.65 µg/ml, respectively. The oil was tested for anticancer activity on human larynx epidermoid carcinoma and colon adenocarcinoma cell lines and found to have cytotoxic activity to both cancerous cell lines, with LC50 of 70 and120 µg/ml, respectively. The results obtained from antibacterial and anticancer tests indicated that essential oils from M. rotunbiloba can be used for the applications in food and pharmaceutical proposes.


Key words: Morus rotunbiloba, essential oil, antibacterial activity, cytotoxicity.