Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3737

Full Length Research Paper

Cytotoxicity and antimicrobial activity of allicin and its transformation products.

Dušica Ilić1, Vesna Nikolić1*, Ana Ćirić2, Marina Soković2, Tatjana Stanojković3, Tatjana Kundaković4, Mihajlo Stanković1 and Ljubiša Nikolić1
1Department of Organic Chemical Technology, Faculty of Technology Leskovac, University of Niš, Serbia. 2Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia. 3Institute for Oncology and Radiology of Serbia, Beograd, Serbia. 4Department of Pharmacognosy, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
Email: [email protected]

  •  Accepted: 22 August 2011
  •  Published: 31 January 2012


Cytotoxicity and antimicrobial activities of allicin and related organo-sulphur products obtained by microwave-assisted transformation of allicin in methanol were studied. The mixture of transformation products of allicin was analysed using liquid chromatography-mass spectrometry (LC-MS) and represents the mixture of ajoenes, vinyldithiins and diallyl disulfide. Cytotoxicity of allicin and its transformation products of allicin were studied against human melanoma FemX and human embryonic lung fibroblast MRC-5 cell lines using MTT test. Allicin and the transformation products of allicin exhibited high selectivity against FemX (IC50 = 0.71 ± 0.20 and IC50 = 0.64 ± 0.52 µg/ml, respectively), while the activity against healthy MRC-5 cells was lower (IC50 = 66.48 ± 3.75 and IC50 = 64.5 ± 1.50 µg/ml, respectively). Allicin has shown strong activity against tested Gram-positive and Gram-negative bacteria and very powerful antifungal activity with minimum inhibitory concentration (MIC) at 0.001 to 0.008 mg × 10-1/ml and MFC at 0.004 to 0.03 mg × 10-1/ml. The transformation products of allicin possessed also very strong antibacterial and antifungal activity, but lower than allicin.


Key words: Cytotoxicity, allicin, organo-sulphur products, antimicrobial activities, liquid chromatography-mass spectrometry (LC-MS).