Cytotoxicity and antimicrobial activities of allicin and related organo-sulphur products obtained by microwave-assisted transformation of allicin in methanol were studied. The mixture of transformation products of allicin was analysed using liquid chromatography-mass spectrometry (LC-MS) and represents the mixture of ajoenes, vinyldithiins and diallyl disulfide. Cytotoxicity of allicin and its transformation products of allicin were studied against human melanoma FemX and human embryonic lung fibroblast MRC-5 cell lines using MTT test. Allicin and the transformation products of allicin exhibited high selectivity against FemX (IC50 = 0.71 ± 0.20 and IC50 = 0.64 ± 0.52 µg/ml, respectively), while the activity against healthy MRC-5 cells was lower (IC50 = 66.48 ± 3.75 and IC50 = 64.5 ± 1.50 µg/ml, respectively). Allicin has shown strong activity against tested Gram-positive and Gram-negative bacteria and very powerful antifungal activity with minimum inhibitory concentration (MIC) at 0.001 to 0.008 mg × 10-1/ml and MFC at 0.004 to 0.03 mg × 10-1/ml. The transformation products of allicin possessed also very strong antibacterial and antifungal activity, but lower than allicin.
Key words: Cytotoxicity, allicin, organo-sulphur products, antimicrobial activities, liquid chromatography-mass spectrometry (LC-MS).
Copyright © 2020 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0