Full Length Research Paper
Abstract
The effect of methyl jasmonate (MJ) on the isoflavonoid accumulation and activities of three antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) were studied in Pueraria mirifica cell suspension culture after MJ elicitation for up to 6 days. Treatment with MJ at a concentration of 1.0 µg/ml induced the highest isoflavonoid contents at the first day after elicitation, when compared to the control. This optimum concentration of MJ induced an oxidative stress in P. mirifica cells. The activity levels of superoxide dismutase and glutathione peroxidase were both increased, whilst that for catalase was decreased in the MJ-treated cells. Thus, P. mirifica cells have a defense mechanism against superoxide and hydrogen peroxide stress and MJ is one such potential stimulator of this defense mechanism.
Key words: Pueraria mirifica, isoflavonoid, methyl jasmonate, superoxide dismutase, catalase, glutathione peroxidase.
Abbreviation
BA, Benzyl adenine; CAT, catalase; GPx, glutathione peroxidase; MJ, methyl jasmonate; MS, Murashige and Skoog’s medium; NAA, naphthylacetic acid; NADPH, nicotinamide adenine dinucleotide phosphate; NBT, nitrotetrazolium blue chloride; ROS, reactive oxygen species; SEM, standard error of the mean; SOD, superoxide dismutase.
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