Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3761

Full Length Research Paper

Protective effects of the active fraction of bitter melon (Momordica charantia) on nitric oxide-induced oxidative stress in LLC-PK1 cell

Eun Ju Cho1, Seung Mi Sin1, Ji Myung Choi1, Sanghyun Lee2, Kye Man Cho3 and Hyun Young Kim3*
1Department of Food Science and Nutrition and Research Institute of Ecology for the Elderly, Pusan National University, Busan 609-735, Korea. 2Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, Korea. 3Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 660-758, Korea.
Email: [email protected]

  •  Accepted: 20 June 2012
  •  Published: 12 September 2012


In this study, in vitro and cellular nitric oxide (NO)-generating systems were used to investigate the NO-scavenging effects of the butanol (BuOH) fraction, the active fraction of bitter melon (Momordica charantia). The BuOH fraction of bitter melon showed NO scavenging activities as concentration-dependent manners. These results suggest that BuOH fraction of bitter melon would be a promising antioxidant through scavenging NO. Furthermore, under LLC-PK1 cellular model, the cells showed declines in viability and increases NO formation against oxidative stress induced by sodium nitroprusside (SNP), generators of NO. However, BuOH fraction of bitter melon significantly and dose-dependently inhibited cell cytotoxicity and NO formation. In addition, the over-expressions of cyclooxygenase-2 and inducible NO synthase induced by SNP were observed, but BuOH fraction of bitter melon down-regulated the expression level of both genes. These results indicate that BuOH fraction of bitter melon would have a protective activity against NO-induced oxidative stress.


Key words: Bitter melon, nitric oxide (NO), LLC-PK1 cell, antioxidative effect, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2).