Abstract

Nine ginsenoside monomers (Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, Rb3, Rd) of ginseng hairy roots and cultivated ginsenoside monomers were simultaneous determined by high performance liquid chromatography (HPLC) to assay chemical components of ginseng hairy roots. The results demonstrated that there were no significant difference between Rg2 of  ginseng hairy roots and  Rg2 of six-year-old cultivated ginseng (P<0.05), and the other eight kinds of ginsenoside monomers from six-year-old cultivated ginseng were higher than that of ginseng hairy roots (P<0.05);  Rg1, Rb2 and Rb3 of  ginseng hairy roots were significantly lower than that of four-year-old cultivated ginseng (P<0.05), and there were no significant difference of other six kinds of ginsenoside monomers from ginseng hairy roots and four-year-old cultivated ginseng (P<0.05). The total content of ginsenosides Rgl and Re were  0.3188%, Rgl and Rb1 0.4848% in ginseng hairy roots, which was comply with quality standards in Chinese Pharmacopoeia (2010) and European Pharmacopoeia, which provide a basis and reference for quality control of ginseng hairy root.

Key words: Ginseng hairy roots, ginseng, phase-high performance liquid chromatography (RP-HPLC), ginsenoside monomer.