Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3761

Full Length Research Paper

Determination of cardiac glycosides in Scilla bifolia (Liliaceae) by two different analytical techniques: Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography - Mass Spectrometry (HPLC-MS)

LUIZA BÄ‚LÄ‚ÅžOIU1, DANIELA CÄ‚LINA2*, ANCA DOCEA2, EMILIA PATRU 2, LAURIAN VLASE1, MARIA VIORICA  BUBULICA2, HONORIUS POPESCU1
1University of Medicine and Pharmacy „Iuliu HaÅ£ieganu”, Faculty of Pharmacy, 8 Victor BabeÅŸ Str, 400012, Cluj-Napoca, România. 2University of Medicine and Pharmacy, 2-4 Petru Rares Str. 200349, Craiova, Romania.
Email: [email protected]

  •  Accepted: 29 October 2013
  •  Published: 10 November 2013

Abstract

In this study was performed chemical analysis on alcoholic extracts of the Scilla bifolia (Liliaceae family) species  harvested from spontaneous flora, with the purpose of determining bioactive cardiac glycosides, particularly proscillaridin A. The used analysis techniques were thin layer chromatography (TLC) and high performance liquid chromatography coupled with mass spectrometry (HPLC-MS). After performing TLC analysis, the presence of the scillarosides was confirmed, but only in traces, in higher amounts in herba than in bulbs. The presence of proscillaridin A has not been determined in any analysed samples, either before or after hydrolysis by HPLC-MS. Thus, we can conclude that, the bulbs and aerial parts of Scilla bifolia do not contain proscillardin A.

Key words: HPLC-MS analysis, alcoholic extract, proscillaridin A, Scilla bifolia, TLC analysis.