Abstract

This study was carried out to evaluate the antioxidant activity of leaves through non-enzymatic method. Tinospora cordifolia (Guduchi) has several beneficial properties, including antioxidant activity. The leaves of guduchi was extracted with methanol and partitioned in water with ethyl acetate and butanol. The phenolic content of the extracts was determined by Folin-Coicalteu method, and antioxidant activity was assayed through non-enzymatic in vitro models such as antioxidant capacity, by radical scavenging activity using1,1-diphenyl-2-picrylhydrazyl  (DPPH) method, reducing power, phosphomolybdenum and metal chelating activity. The phenolic contents of the extracts as gallic acid equivalents were found to be highest in methanol (44.36 mg/g) followed by ethyl acetate (38.73 mg/g), butanol (36.42 mg/g) and water extract (23.60 mg/g). At 250 mg/ml concentration, the antioxidant activity of the free radical scavenging activities of the extracts assayed through DPPH, reducing power, phosphomolybdenum and metal chelating activity were found to be highest with methanol, followed by ethyl acetate, butanol and water extract. The antioxidant activity of BHT was higher than the extracts at each concentration points. The data obtained in the in vitro models clearly establish the antioxidant potency of the extracts from the leaves of T. cordifolia.

Key words: Antioxidant activity, 1,1-diphenyl-2-picrylhydrazyl  (DPPH) activity, metal chelating activity, phosphomolybdenum activity, reducing power, Tinospora cordifolia.