Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Alcohol extract of Pterogyne nitens leaves fails to reduce severity of streptozotocin-induced diabetes in rats

Aline de Souza1, Regina C. Vendramini1, Iguatemy L. Brunetti1, Luis O. Regasini2, Dulce H. Siqueira Silva2, Vanderlan Silva Bolzani2 and Maria T. Pepato1*
1Clinical Analysis Department, School of Pharmaceutical Sciences - UNESP - São Paulo State University. Rua Expedicionários do Brasil n. 1621 - Araraquara, SP, Brazil. 2Organic Chemistry Department, Chemistry Institute - UNESP-São Paulo State University, Rua Francisco Degni s/n - Araraquara, SP, Brasil.
Email: [email protected]

  •  Accepted: 22 April 2010
  •  Published: 04 May 2010

Abstract

Two constituents of Pterogyne nitens leaves, kaempferitrin, a diglycosylated flavonol, and galegin, a guanidine alkaloid, may be considered likely to exert an antidiabetic effect, on the basis of their chemical structures. Thus, experimentally diabetic rats were treated with P. nitens leaf extract, to observe the effects on biochemical and toxicological marker variables. Streptozotocin-diabetic rats (50 mg/kg body weight) were given ethanolic extract of the leaves (76 mg suspended in 0.5 mL of 10% aqueous glycerine per rat) (DP) by gavage, twice a day for 32 days. Diabetic controls were given 0.5 mL of 10% glycerine (DG), insulin (2.5 U in 0.3 mL s. c.) (DI) or 0.5 mL water (DW). Initial glycemia was 537.11 ± 10.35 mg/dL.  Each week or fortnight after the treatment glucose, urea and protein contents were determined in the urine and glycemia and alkaline phosphatase activity in the serum. Except for proteinuria, the results for groups DP, DG and DW all differed significantly (p < 0.05) from those for group DI, which exhibited reduced values of all the other variables. The plant extract neither improved nor worsened the diabetic state of the rats; nor did it give rise to any hepato-biliary toxic effect.

 

Key words: Antidiabetic plant, alkaline phosphatase, biochemical markers, hepato-biliary toxicity marker.