Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3693

Full Length Research Paper

Anti-HIV-1 activity in human primary cells and Anti-HIV-1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera

Caio Cesar Richter Nogueira
  • Caio Cesar Richter Nogueira
  • Departamento de Biologia Marinha, Instituto de Biologia, Universidade Federal Fluminense, P. O. Box 100.644, 24001-970, Niterói, RJ, Brazil.
  • Google Scholar
Izabel Christina Nunes de Palmer Paixão
  • Izabel Christina Nunes de Palmer Paixão
  • Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, 24020-141, Niterói, RJ, Brazil.
  • Google Scholar
Claudio Cesar Cirne-Santos
  • Claudio Cesar Cirne-Santos
  • Laboratório de Imunologia Clínica, Instituto Oswaldo Cruz, FIOCRUZ, 21.040-900, Rio de Janeiro, RJ, Brazil.
  • Google Scholar
Paulo Roberto Soares Stephens
  • Paulo Roberto Soares Stephens
  • Laboratório de Inovações em Terapias, Ensino e Bioprodutos, Instituto Oswaldo Cruz, FIOCRUZ, 21.040-900, Rio de Janeiro, RJ, Brazil.
  • Google Scholar
Roberto Campos Villaça
  • Roberto Campos Villaça
  • Departamento de Biologia Marinha, Instituto de Biologia, Universidade Federal Fluminense, P. O. Box 100.644, 24001-970, Niterói, RJ, Brazil.
  • Google Scholar
Helena de Souza Pereira
  • Helena de Souza Pereira
  • Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, 24020-141, Niterói, RJ, Brazil.
  • Google Scholar
Valéria Laneuville Teixeira
  • Valéria Laneuville Teixeira
  • Departamento de Biologia Marinha, Instituto de Biologia, Universidade Federal Fluminense, P. O. Box 100.644, 24001-970, Niterói, RJ, Brazil.
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  •  Received: 22 July 2016
  •  Accepted: 06 September 2016
  •  Published: 17 September 2016

Abstract

First generation drugs such as zidovudine have been extensively used in clinical practice, resulting in the development of HIV resistance to these nucleoside analogs. Several studies have demonstrated the effective anti-HIV activity of natural products derived from seaweeds, suggesting promising sources of substances for the development of novel antiviral drugs. In this paper, the antiviral effect of extracts from the red seaweed Acanthophora spicifera on HIV-1 replication was evaluated in vitro. Peripheral blood mononuclear cells obtained using the Ficoll-Hypaque gradient were used for cytotoxicity and antiviral activity testing. The dichloromethane extracts, ethyl acetate, acetone, and methanol were found to have CC50 values of 31±7.4, 45±11, 38±3.5, and 179±25 µg/mL, respectively. With the control, the extract prepared in ethyl acetate inhibited approximately 60% of the viral load, which is the best result among the extracts. This same extract showed an IC50 value of 33.17±4.84 μg/mL for the reverse transcriptase. The EtOAc extract from A. spicifera showed to be an efficient HIV antiviral due to its phenolic compounds, as evaluated by nuclear magnetic resonance.

Key words: Marine natural products, red seaweed, Acanthophora spicifera, HIV-1, Antiviral activity, Anti-HIV-1 RT.