Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3831

Short Communication

Effect of different pretreatment on callus formation from anther in balsam pear (Momordica charantia L.)

Yi Tang, Xiaomei Li, Juan Li, Chao Ma, Jia Lai and Huanxiu Li*
College of Horticulture, Sichuan Agricultural University, Ya’an 62504, Sichuan, China.
Email: [email protected]

  •  Accepted: 26 January 2012
  •  Published: 09 May 2012

Abstract

The effect of different pretreatment on callus induction from anther in three cultivars of balsam pear (Momordica charantia L.) was investigated. The results showed that cold pretreatment was the best for anther callus induction, and followed by heat shock, while centrifugation had no significant effect on callus induction from anther. Cold pretreatment for 24 h proved to be optimal as it significantly increased the callus induction rate, which varied with cultivars, reaching the maximum of 44.00, 51.10 and 72.67% for cv. Lvcui, Cuifei and Bixiu, respectively.

 

Key words: Balsam pear (Momordica charantia L.), anther culture, callus induction, pretreatment.

Abbreviation

Abbreviations: 2,4-D, 2,4-dichlorophenoxyacetic acid; 6-BA, 6-benzyladenine; MS,Murashige and Skoog (1962).

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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