Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3835

Full Length Research Paper

Aerial parts of Solanum agrarium Sendtn. (Solanaceae) present the flavonoid myricetin 3,7,3’ trimethyl ether and antispasmodic effect on guinea-pig ileum by blockade of voltage-gated calcium channels

Ana Carolina de Carvalho Correia1*, Cibério Landim Macêdo1, Fabio de Souza Monteiro1, Gislaine Alves de Oliveira1, Rosimeire Ferreira dos Santos2, Roberto Jefferson Bezerra do Nascimento3, Celso de Amorim Camara4, Maria de Fátima Agra1,6, Tania Maria Sarmento da Silva5, Bagnólia Araújo da Silva1,6
1Pós-Graduação em Produtos Naturais e Sintéticos Bioativos / Centro de Ciências da Saúde (CCS)”, Universidade Federal da Paraíba, P.O. Box 5009, 58051-970, João Pessoa-PB, Brazil. 2Departamento de Bioquímica e Farmacologia, Universidade Federal do Piauí, Campus Universitário Ministro Petrônio Portella, CEP 64049-550 , Teresina – PI, Brazil. 3Colegiado de Zootecnia, Universidade Federal do Vale do São Francisco, Rod. BR 407 Km 12 Lote 543 Projeto de Irrigação Senador Nilo Coelho, s/no - C1. CEP 56300-000 – Petrolina – PE – Brazil. 4Departamento de Ciências Moleculares, Universidade Federal Rural de Pernambuco, Campus Dois Irmãos s/n, CEP 52171-980, Recife-PE, Brazil. 5Departamento de Química, Universidade Federal Rural de Pernambuco, Campus Dois Irmãos s/n, CEP 52171-980, Recife-PE, Brazil. 6Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba, P.O. Box 5009, 58051-970, João Pessoa-PB, Brazil.
Email: [email protected]

  •  Accepted: 26 July 2013
  •  Published: 17 August 2013

Abstract

Solanum agrarium Sendtn. is popularly known in Northeastern Brazil as "gogóia". Previous work has verified the presence of the flavonoid kaempferol and showed the crude ethanolic extract from aerial parts of S. agrarium (SA‑EtOH) to have non-selective antispasmodic effect on guinea‑pig ileum. The aim was to isolate and identify the special metabolites and to investigate antispasmodic mechanism of SA‑EtOH on guinea-pig ileum. From their aerial parts was isolated the flavonoid myricetin‑3,7,3’‑trimethyl ether. SA‑EtOH shifted to the right cumulative concentration-response curves to carbachol or histamine in a reversible and non-competitive manner. SA‑EtOH relaxed the ileum pre-contracted with 40 mM KCl (EC50=17.4±3.5 µg/ml), 10‑6 M carbachol (EC50=119.4±24.3 µg/ml) or 10‑6 M histamine (EC50=18.7±4.6 µg/ml). This is most likely due to the inhibition of calcium influx through voltage-gated calcium channels (CaV). It was confirmed by SA‑EtOH antagonizing CaCl2-induced contractions in depolarizing medium nominally without Ca2+, with non‑parallel shift of the concentration-response curves to the right and reduction of Emax. The uncovering of SA‑EtOH (EC50=187.2±27.3 µg/ml) inhibiting tonic contractions induced by S‑(‑)‑BayK8644 suggests that Ca2+ channel subtype involved is CaV-L. In conclusion, SA‑EtOH shows antispasmodic effect on guinea-pig ileum due, in part, to blockade of calcium influx through CaV channels.

 

Key words: Solanum agrarium, flavonoid, antispasmodic effect, guinea-pig ileum, CaV-L.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 7 No. 31
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES