Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3830

Full Length Research Paper

Genetic diversity of wild Auricularia polytricha in Yunnan Province of South-western China revealed by sequence-related amplified polymorphism (SRAP) analysis

P. Du1,2, B. K. Cui1 and Y. C. Dai1*
1Institute of Microbiology, P. O. Box 61, Beijing Forestry University, Beijing, 100083, P. R. China. 2Heilongjiang Agricultural Economy Vocational College, Mudanjiang, 157041, P. R. China.
Email: [email protected]

  •  Accepted: 08 February 2011
  •  Published: 18 April 2011

Abstract

As an economically and medically important fungus, Auricaularia polytricha has been collected and cultivated widely in China, however, its diversity has not yet investigated previously. The genetic diversity of 20 wild A. polytricha strains from Yunnan Province and four cultivated strains from Henan and Sichuan Province were profiled by using 10 pairs of sequence-related amplified polymorphism (SRAP) primers. A dendrogram tree was constructed by unweighted pair-group method with arithmetic means (UPGMA) method according to the similarity coefficient among strains. A total of 425 (P=99.8%) and 37 (P=40.7%) polymorphic loci were detected in the wild and cultivated strains respectively, and the 24 strains were classified into six groups with the similarity coefficient of 0.74. The genetic diversity of the wild strains was much higher than that of the cultivated strains, and SRAP marker was an effective method to evaluate the genetic diversity of A. polytricha.

 

Key words: A total of 425 (P=99.8%), 37 (P=40.7%) polymorphic loci.