Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3786

Full Length Research Paper

Analgesic, anti-inflammatory and antipyretic activities from flavonoid fractions of Chromolaena odorata

Bamidele Victor Owoyele1*, Stephen Olubunmi Oguntoye2, Kemi Dare1, Bolatito Alice Ogunbiyi1, Elizabeth Adeola Aruboula1 and Ayodele Olufemi Soladoye1    
1Department of Physiology, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin, P. M. B. 1515, Ilorin, Nigeria. 2Department of Chemistry, Faculty of Sciences, University of Ilorin, P. M. B. 1515, Ilorin, Nigeria.      
Email: [email protected],[email protected]

  •  Accepted: 16 September 2008
  •  Published: 28 September 2013

Abstract

The ethanolic extract of Chromolaena odorata was fractionated with solvent-solvent extraction technique using the following solvents successively, n-hexane, dichloromethane, ethyl acetate, n-butanol and water. The fractions were evaluated for analgesic, anti-inflammatory and antipyretic activities using standard experimental models which includes; hot plate and formalin paw licking tests for analgesic activities, carrageenan paw oedema and cotton pellet granuloma for anti-inflammatory activities and Brewer’s yeast induced pyrexia for antipyretic tests. The dichloromethane (DCF), n-butanol (nBF) and ethyl acetate (EAF) fractions were analyzed using analytical thin-layer chromatography (TLC), and preparative thin-layer chromatography (PTLC). Spectral studies were carried out using ultra-violet (UV) and infra-red (IR) spectroscopy. Phytochemical screening was carried out on the isolated compounds and the Rf  values were determined. The result shows that the DCF produced consistent analgesic, anti-inflammatory and antipyretic activities followed by the nBF and EAF. Spectroscopic and phytochemical analyses revealed the presence of flavonoid in DCF. Flavonoids were also detected in nBF and EAF. The biological activities of the extract can therefore be attributed to the presence of flavonoids in the fractions.

 

Key words: Analgesic, anti-inflammatory, antipyretic, Chromolaena odorata, flavonoids, fractions.