Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3785

Full Length Research Paper

Phytochemistry and in vitro anti-oxidant activities of Stellaria media, Cajanus cajan and Tetracera potatoria methanolic extracts

Oyebanji, Olanike Bukola1 and Saba, Adebowale Bernard2*
1Department of Animal Science, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria. 2Department of Veterinary Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Nigeria.
Email: [email protected], [email protected]

  •  Accepted: 11 May 2011
  •  Published: 16 December 2011

Abstract

Phytochemical screening and determination of antioxidant activities of methanolic extracts of leaf of Stellaria mediaCajanus cajan and root of Tetracera potatoria were conducted. Phytoconstituents screened for included alkaloid, saponins, tannins, phlobatannins, flavonoids, anthraquinones and cardiac glycosides. Free radicals scavenging capacity of the extract of the three plants were assessed in vitro by 1,1 diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and ferric reducing antioxidant power (FRAP) assays, while inhibition of free radicals generation was assessed by ability of the extracts to inhibit lipid peroxides formation. The antioxidant activities of the extracts were dose dependent. The highest percentage inhibition of DPPH by TpotatoriaSmedia and Ccajan was 82, 76 and 57%, respectively, as against 76% for ascorbic acid. Percentage ferric ions reduction in FRAP assay was 104, 79 and 26% for TpotatoriaSmedia and Ccajan as against 100% for Gallic acid, the standard compound. The standard drugs however appeared more potent than the crude extracts because the bioactive compounds have not been isolated or purified. Lipid peroxidation assay showed that Tpotatoria inhibited lipid peroxides formation by 54%. It was concluded that the abundance of flavonoids, tannins and phlobatannins in the three plants account for their high antioxidant activities which probably explains their ethnomedical applications against inflammatory conditions. It is believed that isolation of the specific bioactive principles will serve to improve the free radicals scavenging potency of the compounds.

 

Key words: Free radicals, phytoconstituents, Stellaria mediaCajanus cajanTetracera potatoria, extracts.