Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3804

Full Length Research Paper

Chemotaxis movement assay of Eurycoma longifolia using wild and disarmed strains of Agrobacterium rhizogenes

Monica Danial1, Chan Lai Keng1, Syarifah Shahrul Rabiah Syed Alwee2, Maziah Mahmood3 and Sreeramanan Subramaniam1*
1School of Biological Sciences, Universiti Sains Malaysia, 11800 Gelugor, Penang, Malaysia. 2Felda Biotechnology Centre, Felda Agriculture Services Sdn. Bhd. Tingkat 7, Balai Felda, Jalan Gurney 1,54000 Kuala Lumpur, Malaysia. 3Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Selangor, Malaysia.
Email: [email protected]

  •  Accepted: 16 December 2010
  •  Published: 18 April 2011


Bacterial chemotaxis is considered the first step in the interaction between motile bacteria and plant cells. Chemotaxis initiates the process of bacterial infection towards the plant cells and thus conferring beneficial attributes to the host. In this study, 5 wild strains and 2 disarmed strains of Agrobacterium rhizogenes were tested for chemotaxis assay using the swarm agar plate method. As expected, strong positive chemotactic response was observed in most of the tested bacteria strains and all the tested strains of Agrobacterium rhizogenes showed positive chemotactic response towards the tested root and somatic embryos of the valuable medicinal plant, Eurycoma longifolia. Therefore, induction of hairy roots is possible in Eurycoma longifolia. Generating hairy roots in Eurycoma longifolia will be highly beneficial mainly to the pharmaceutical industry as this medicinal plant possesses the capacity to produce many secondary metabolites which is proposed to increase sexual virility properties and to have anti cancer properties.


Key words: Eurycoma longifolia, chemotactic movement, Agrobacterium rhizogenes,roots, somatic embryos.