Abstract

An efficient plant regeneration system has been developed from nodal segments of Naringi crenulata (Roxb.) Nicolson used as a medicinal plant. Nodal segments, after being sterilized with 0.1% mercuric chloride were inoculated in Murashige and Skoog’s (MS) medium with varied concentrations of BAP, Kn and their combinations. Different parameters including frequency, number of shoots per explant, length of shoots (cm) were studied during the course of the investigation. Addition of adenine sulphate (25.0 mg/L) and glutamine (150.0 mg/L) to the medium was found beneficial for maximum shoot bud induction. Maximum numbers of shoots (18.8 ± 0.663) were obtained on MS medium supplemented with BAP (2.0 mg/L) along with additives. Best elongation of the shoot buds was obtained on MS medium supplemented with BAP (0.5 mg/L) and Kn (0.5 mg/L) along with additives. Elongated shoots were isolated and cultured on ½ strength MS medium supplemented with different concentrations of IAA, IBA and NAA for root induction. Highest degree of rooting was obtained on MS medium supplemented with IBA (1.0 mg/L). The rooted plants were transplanted to pots for hardening. This is the first report dealing with in vitro rapid multiplication for N. crenulata.

Key words: Naringi crenulata, nodal explants, multiplication, rooting.

Abbreviation

BAP, - 6-benzylaminopurine; NAA, - α-naphthaleneacetic acid; Kn, – kinetin, IBA, - indole-3-butyric acid, IAA, – indole-3-acetic acid.