Full Length Research Paper
Abstract
The extract of Coptidis Rhizoma was determined antifungal activity against five differentCandida species. The extract showed antifungal activity against Candida albicans at 200 μg/ml, and C. tropicalis and C. glabrata at 50 μg/ml. A compound 1 was separated by thin layer chromatography (TLC) analysis and confirmed as berberine by high performance liquid chromatography (HPLC) analysis. The minimum inhibitory concentration (MIC) values of the compound 1 and berberine were 24 and 48 μg/ml against C. tropicalis and C. glabrata, and 30 and 50 μg/ml against C. tropicalis and C. glabrata. The IC50 values of the compound 1 were 58 μM at 24 h and 40 μM at 48 h by MTT assay of cell viability using HaCaT cell line. The above results indicated that berberine was a good candidate to inhibit the propagation ofC. tropicalis and C. glabrata, even in human skin.
Key words: Anticandidial activity, coptidis rhizoma, MTT assay, HaCaT cell line.
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