Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3833

Full Length Research Paper

Phytochemical screening and antibacterial activity of Citrullus colocynthis (Linn.) Schrad against Staphylococcus aureus

Shahla Najafi1, Nima Sanadgol1*, Batool Sadeghi Nejad2, Maryam Ashofteh Beiragi3 and Ehsan Sanadgol4
1Department of Biology, Faculty of Science, Zabol University, Zabol, Iran. 2Department of Mycoparasitology, Jundishapur university of Medical Sciences, Ahvaz, Iran. 3Department of Agronomy and Plant Breeding, College of Agriculture, Zabol University, Zabol, Iran. 4School of Pharmacy, Mashhad University of medical science, Mashhad, Iran.
Email: [email protected], [email protected]

  •  Accepted: 18 August 2010
  •  Published: 18 November 2010


Citrullus colocynthis (Linn.) Schrad (CCT) is an important medicinal plant belonging to the family of Cucurbitaceae. It is a well recognized plant in the traditional medicine and was used by people in rural areas as a purgative, antidiabetic and insecticide. In the present investigation, after phytochemical screening of CCT through standard experimental procedure, antibacterial properties of this plant were studied. The antibacterial activity of CCT leaves and fruits extracts (water and ethanolic) against standard (ATCC 25923) and hospitalisolated strains of Staphylococcus aureus from novobiocin treatment patients were evaluated using disc diffusion method. The inhibitory effects of this extracts were compared with standard antibiotic, novobiocin. Phytochemical screening of CCT revealed the presence of tannins, saponins, alkaloids, flavonoides and glycosides. The ethanolic extract showed inhibitory activity against S. aureus more than water extract and this effect was dose dependent manner. Results indicated that 5 mg/mL fruits ethanolic extract have a similar inhibitory effect with novobiocinagainst standard strain. We suggest one of the chemical components that exist in ethanolic extract such as alkaloids, flavonoides and glycosides can have a powerful antibacterial effect even more than novobiocin, especially against hospital isolated strains. The study scientifically validates the use of plant materials in traditional medicine.


Key words: Citrullus colocynthisStaphylococcus aureus, phytochemical screening.