Journal of
Medicinal Plants Research

  • Abbreviation: J. Med. Plants Res.
  • Language: English
  • ISSN: 1996-0875
  • DOI: 10.5897/JMPR
  • Start Year: 2007
  • Published Articles: 3693

Full Length Research Paper

Elicitation induced flavonoids, phenolic constituents, antioxidant and cytotoxic activities of Artemisia monosperma callus cultures

Amal Amin Al-Gendy
  • Amal Amin Al-Gendy
  • Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
  • Google Scholar
Rabab Morad Ali
  • Rabab Morad Ali
  • Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
  • Google Scholar
Dalia Ibrahim Hamdan
  • Dalia Ibrahim Hamdan
  • Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
  • Google Scholar
Afaf El-Sayed Abdel-Ghani
  • Afaf El-Sayed Abdel-Ghani
  • Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt.
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  •  Received: 05 August 2016
  •  Accepted: 11 October 2016
  •  Published: 25 October 2016

Abstract

Artemisia monosperma (Delile) callus was induced using seedling explant cultured on Murashige and Skoog solid medium (M&S) supplemented with 1 mg/L naphthalene acetic acid (NAA) and 1 mg/L kinetin (Kn). Cultures were maintained on 2 mg/L 2,4-dichlorophenoxy acetic acid (2, 4- D) and 1 mg/L Kn and treated with different elicitors. Total flavonoids and phenolics were determined by aluminum chloride-potassium acetate and Folin-Ciocalteu colorimetric methods, respectively. Yeast extract 10 mg/L (Y2) showed higher productivity and viability of callus cultures than Fusarium oxysporum and calcium chloride elicitors. Y2 shows an increase of 2.4 and 1.5 times non-elicited calli for flavonoids and phenolic compounds production, respectively. Pockets of embryogenic calli were transferred to M&S solid media supplemented with 0.5 mg/L NAA and 0.5 mg/L benzyl aminopurine (BAP), followed by hormonal free media where different stages of embryos were monitored, giving regenerated plantlets. HPLC analysis of methylene chloride and ethyl acetate fractions of parent plant (P), Y2 elicited embryogenic callus (EC.Y2) and Y2 elicited non-embryogenic callus (NEC.Y2) showed that, quercetin content in methylene chloride fraction of NEC.Y2 is 7.2 times the same fraction of P, while vanillic acid ethyl ester content in ethyl acetate fraction of EC.Y2 (EEC.Y2) is 8.6 times the same fraction of P. EEC.Y2 showed highest anti-oxidant activity with IC50 7.22±0.14 µg/mL compared with ethyl acetate fraction of P with IC50 20.01±0.82 µg/mL (IC50 of L-ascorbic acid = 1.24±0.07 µg/mL). Using MTT assay, EEC.Y2 exhibited potent cytotoxic activity against colon carcinoma cells and moderate activity against hepatocellular and lung carcinoma cells with IC50 18.9±1.4, 22.3±0.9 and 41.6±1.2 µg/mL, respectively; compared with doxrubcin as reference standard.

 

Key words: Artemisia monosperma, callus cultures, flavonoids, phenolic compounds, anti-oxidant, cytotoxicity.