August 2012
Cellular antioxidant and antiproliferative activity of ethanolic extract of Coleus tuberosus
Ethanolic extract of both the flesh and peel of Coleus tuberosus (EEPC) were evaluated for cellular antioxidant activity based on 2,7,-diacetate dichlorofluorescein (DCFH) oxidation, and antiproliferative activity based on 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT) assay, using human breast cancer MCF-7 cells. The result showed that antioxidant and antiproliferative activity of EEPC was...
August 2012
Nuclear DNA content and genome size of American ginseng
Flow cytometry analysis of propidium iodide (PI) stained nuclei isolated from leaf tissues was used to estimate the genome size of American ginseng (Panax quinquefolius L.) and evaluate inhibitory effects of its secondary compounds on PI intercalation. American ginseng nuclear DNA content was estimated to be 10.05 ± 0.04 pg/2C, and therefore its haploid (1C) genome size is 4914 Mbp. There was no significant...
August 2012
Total phenolic contents and antioxidant activities of pomegranate peel, seed, leaf and flower
Pomegranate (Punica granatum L.) is a nutrient dense food rich in beneficial phytochemicals. The aim of this study is to investigate and to determine antioxidant contents from local and highly consumed pomegranate variety called ‘Gabsi’. Peels, seeds, leaves and flowers were used to quantify total polyphenols, flavonoids, anthocyanins and hydrolysable tannins. Antioxidants contents were...
August 2012
Effect of different extracts of Stevia rebaudiana leaves on Streptococcus mutans growth
Plants produce a diverse range of bioactive molecules, making them rich source of different type of medicines. In this study, effect of aqueous, methanol, ethanol and acetone extracts of Stevia rebaudiana leaves were evaluated against Streptococcus mutans. Agar-well diffusion method was used to determine the antibacterial activity of extracts. Minimal inhibitory concentration was obtained by...
August 2012
Mineral contents and antioxidant potential of selected legumes of Pakistan
Legumes are an important component of the common mans’ diet in Pakistan and various species of legumes are grown throughout Pakistan. Little work exists on determination of metal contents and antioxidant activity of pods and leaves of food legumes. Current research is designed to quantify metal contents of leaves and pods of selected legumes and to establish antioxidant profiles of crude methanolic extracts of...
August 2012
Assessment of genetic diversity in two Allium spp. using random amplified polymorphic DNA markers
Genetic diversity was estimated among fifteen local cultivars of two Allium species (14 ofAllium cepa and 1 Allium ascalonicum) using 6 random amplified polymorphic DNA (RAPD) primers. A total of 54 RAPD amplicons were obtained, of which 25 (46.3%) were polymorphic. The percent polymorphism between primers ranged from 6.9 to 30.2%. The highest genetic distance (75%) was observed between light...
August 2012
Real time sequence characterized amplified region (RT-SCAR) marker: Development and its application for authentication and quantification of Catharanthus roseus L. Don.
The objective of the present study was to develop a Real Time-Sequence Characterized Amplified Region (RT-SCAR) of Catharanthus roseus, an effective molecular marker for authentication and quantification. Here, we made a comparative study of SCAR and RT-SCAR marker. These newer RT-SCAR methods are useful for the correct identification and quantification of C. roseus. In particular, this technique is very...
August 2012
Analysis of and correlation between phytochemical and antimicrobial constituents of Ceriops decandra, a medicinal mangrove plant, from Indian Sundarban estuary
The present study was aimed at screening the phytochemical contents and antimicrobial activities of different solvent extracts as well as partial identification of the bioactive constituents from leaf, root, wood and bark of Ceriops decandra, a mangrove plant from Sundarban estuary, India. Phytochemical analysis revealed the presence of high phenolics (GAE 42.33 - 163.44 mg/g), flavonoids (QE 39.16 - 582.22...
August 2012
Phytochemical profile and antibacterial activity of bark and leaves of Caesalpinia pyramidalis Tul. and Sapium glandulosum (L.) Morong.
This study aimed to analyze the phytochemical profile and antibacterial activity of crude ethanol extracts (CEE) from the bark and leaves of Caesalpinia pyramidalis Tul. andSapium glandulosum (L.) Morong. The phytochemical composition was analyzed by thin layer chromatography (TLC) using eluent and specific revealing. Antibacterial activity was evaluated using the agar diffusion method (well technique)...
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