Journal of
Plant Breeding and Crop Science

  • Abbreviation: J. Plant Breed. Crop Sci.
  • Language: English
  • ISSN: 2006-9758
  • DOI: 10.5897/JPBCS
  • Start Year: 2009
  • Published Articles: 409

Full Length Research Paper

Biochemical and molecular characterization of submergence tolerance in rice for crop improvement

Bishun D. Prasad1,4*, Ganesh Thapa2,3, Samindra Baishya2 and Sangita Sahni4
  1Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, Assam, India. 2Department of Biochemistry, Assam Agricultural University, Jorhat, Assam, India. 3Department of Biotechnology, Indian Institute of Technology Guwahati, Assam, India. 4Depatment of Biology, the University of Western Ontario, ON, Canada N6A 5B7, Canada
Email: [email protected]

  •  Published: 30 September 2011

Abstract

 

Submergence stress is considered as the third most important limitation of rice production contributing to its low productivity in lowland and rainfed ecosystem. Characterization of genotypes and using them in breeding programme is likely the best option to withstand submergence and stabilize productivity in these environments. However, progress in genotypes characterization has been slow but can substantially be enhanced by using potential of biochemical and molecular markers, to enhance and speedup progress through breeding. Survival percentage, alcohol dehydrogenase (ADH) activity and isozyme profiles were carried out in this investigation. The response to submergence appears to be complex and involves a number of enzymes, therefore, cultivars were also characterized by using other isozymes (Aspartate aminotransferase, AAT; Malate dehydrogenase, MDH; Esterase, EST and Peroxidase, POX) and molecular marker like random amplified polymorphic DNA (RAPD). Dendrogram constructed, using isozymes and RAPD data clustered the rice genotypes into three major groups, submergence tolerant, moderately tolerant and susceptible. The result obtained in this study will help plant breeders in breeding high yielding cultivars for lowland eco-systems, with submergence tolerant cultivars.

 

Key words: Rice, submergence stress, alcohol dehydrogenase (ADH), isozyme, random amplified polymorphic DNA (RAPD), FR13A.