Journal of
Plant Breeding and Crop Science

  • Abbreviation: J. Plant Breed. Crop Sci.
  • Language: English
  • ISSN: 2006-9758
  • DOI: 10.5897/JPBCS
  • Start Year: 2009
  • Published Articles: 409

Full Length Research Paper

Estimation of heterosis and combining ability in maize (Zea mays L.) for maize lethal necrosis (MLN) disease

Kiyyo J. G.
  • Kiyyo J. G.
  • Ilonga Agricultural Research Institute, P. O. Box 33 Kilosa, Tanzania.
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Kusolwa P. M.
  • Kusolwa P. M.
  • Department of Crop Science and Horticulture, Sokoine University of Agriculture, P. O. Box 3005, Morogoro, Tanzania.
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  •  Received: 21 March 2017
  •  Accepted: 28 June 2017
  •  Published: 30 September 2017


Nature of gene action and genetic parameters for disease resistance are important attributes in developing resistant cultivars. This provides the sustainable, economically justifiable and environmentally friendly means of controlling plant diseases. In this study 6 x 6 full diallel cross involving genetically divergent maize inbred lines was performed with the aim of developing resistant cultivars against Maize Lethal Necrosis (MLN) disease under MLN disease hot spot areas in Mlangarini, Ngaramtoni and Kiru six in the Northern Zone of Tanzania during 2015 cropping season. The experimental materials consisted of thirty single cross hybrids, six parents and two local checks. The experiment was laid down in a randomized complete block design (RCBD) with three replications per location. The general combing ability (GCA) and specific combining ability (SCA) effects were significantly different for MLND response among genotypes across all locations. The combined analysis revealed that GCA was highly significant at (P≤0.001) than SCA in all locations with mean squares of (5.551***), (1.61***) and (4.527***) for Mlangarini, Kiru six and Ngaramtoni respectively. The GCA: SCA ratios were 1.894, 1.726 and 1.403 for Mlangarini, Kiru six and Ngaramtoni respectively. The implication of GCA/SCA ratio of more than a unity proves that GCA is significant in all locations where this study was conducted. The results also revealed the presence of both additive and non-additive genetic effects, with the former more pronounced than the later. This implies that developing composite variety will be the better option in combating the disease. However the best cross was observed between CML 144 X CML444 with mean square -0.10, -0.45* and -0.18* for Mlangarini, Kiru six and Ngaramtoni respectively.


Key words: Diallel cross, general combing ability (GCA), heritability, Maize Lethal Necrosis disease (MLND), specific combining ability (SCA), Zea mays.