Journal of
Plant Breeding and Crop Science

  • Abbreviation: J. Plant Breed. Crop Sci.
  • Language: English
  • ISSN: 2006-9758
  • DOI: 10.5897/JPBCS
  • Start Year: 2009
  • Published Articles: 409

Full Length Research Paper

Differential morphological, physiological, and molecular responses to water deficit stress in sugarcane

Cássia Ângela Pedrozo
  • Cássia Ângela Pedrozo
  • Center of Agroforestry Research of Roraima. Brazilian Agricultural Research Corporation ? EMBRAPA, Rodovia BR-174, km 8, Distrito Industrial, zip code: 69301-970, Boa Vista, Roraima, Brazil.
  • Google Scholar
John Jifon
  • John Jifon
  • Texas A&M Agrilife Research and Extension Center, the Texas A&M System, 2415 E Highway 83, Zip Code: 78596, Weslaco, Texas, United States.
  • Google Scholar
Márcio Henrique Pereira Barbosa
  • Márcio Henrique Pereira Barbosa
  • Federal University of Viçosa, Departamento de Agronomia, Avenida P.H. Rolfs, zip code: 36570-000, Viçosa, Minas Gerais, Brazil.
  • Google Scholar
Jorge Alberto Da Silva
  • Jorge Alberto Da Silva
  • Texas A&M Agrilife Research and Extension Center, the Texas A&M System, 2415 E Highway 83, Zip Code: 78596, Weslaco, Texas, United States.
  • Google Scholar
Jong-Won Park
  • Jong-Won Park
  • Texas A&M Agrilife Research and Extension Center, the Texas A&M System, 2415 E Highway 83, Zip Code: 78596, Weslaco, Texas, United States.
  • Google Scholar
Nora Solis Gracia
  • Nora Solis Gracia
  • Texas A&M Agrilife Research and Extension Center, the Texas A&M System, 2415 E Highway 83, Zip Code: 78596, Weslaco, Texas, United States.
  • Google Scholar


  •  Received: 23 January 2015
  •  Accepted: 30 June 2015
  •  Published: 28 July 2015

Abstract

This study was designed to characterize morphological, physiological and molecular responses of sugarcane genotypes to a simulated water deficit stress. Two genotypes (TSP05-4: Drought-tolerant; TCP02-4589: Drought-sensitive) were subjected to a 20-day water deficit treatment and an 8-day recovery period. Leaf photosynthesis (Pn), transpiration rate (E), stomatal conductance (Gs), leaf greenness index (SPAD) and variable-to-maximum chlorophyll a fluorescence ratio (Fv/Fm), were evaluated before, during and after water deficit. Root-to-shoot ratio (R/S), stalk height (SH), diameter (SD) and stalk weight (SW) were evaluated at the end of the experiment. Real-time RT-PCR confirmed seven differentially-expressed transcript-derived fragments (TDFs) identified by cDNA-AFLP. Pn rates were similar between the genotypes under well-watered conditions. However, under water deficit, TSP05-4 had higher Pn rates. SPAD, Fv/Fm and R/S were also generally higher in TSP05-4, regardless of soil moisture status. Water deficit-induced reductions in SH and SW were greater in TCP02-4589 than in TSP05-4. Three TDFs showing sequence similarities to genes encoding a putative expressed pentatricopeptide, a protein kinase CK2 regulatory subunit CK2β3, and a glucose-6-phosphate/phosphate translocator 2 were identified in TCP02-4589. One TDF similar to a drought-inducible protein was identified in TSP05-4. Recovery of physiological processes and gene expression patterns to the water stress levels was fast.

 

Key words: Differential gene expression, water deficit stress, re-watering,   Saccharum spp.