Journal of
Plant Breeding and Crop Science

  • Abbreviation: J. Plant Breed. Crop Sci.
  • Language: English
  • ISSN: 2006-9758
  • DOI: 10.5897/JPBCS
  • Start Year: 2009
  • Published Articles: 421

Full Length Research Paper

Inheritance and detection of QTL in cowpea resistance to brown blotch disease

Ibié G. THIO
  • Ibié G. THIO
  • Laboratory of Genetic and Plant Biotechnology, Institut de l’Environnement et de Recherches Agricoles (INERA), Ouagadougou 01 BP 476, Burkina Faso. 2Laboratory of Plant Pathology, Institut de l’Environnement et de Recherches Agricoles (INERA), Ouagadougou 01 BP 476, Burkina Faso
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Jean Baptiste TIGNEGRE
  • Jean Baptiste TIGNEGRE
  • Laboratory of Genetic and Plant Biotechnology, Institut de l’Environnement et de Recherches Agricoles (INERA), Ouagadougou 01 BP 476, Burkina Faso.
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Inoussa DRABO
  • Inoussa DRABO
  • Laboratory of Genetic and Plant Biotechnology, Institut de l’Environnement et de Recherches Agricoles (INERA), Ouagadougou 01 BP 476, Burkina Faso.
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Joseph T. B. BATIENO
  • Joseph T. B. BATIENO
  • Laboratory of Genetic and Plant Biotechnology, Institut de l’Environnement et de Recherches Agricoles (INERA), Ouagadougou 01 BP 476, Burkina Faso.
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Elisabeth P. ZIDA
  • Elisabeth P. ZIDA
  • Laboratory of Plant Pathology, Institut de l’Environnement et de Recherches Agricoles (INERA), Ouagadougou 01 BP 476, Burkina Faso
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Mahamadou SAWADOGO
  • Mahamadou SAWADOGO
  • Laboratory of Genetic and Plant Biotechnology, Institut de l’Environnement et de Recherches Agricoles (INERA), Ouagadougou 01 BP 476, Burkina Faso.
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Paco SEREME
  • Paco SEREME
  • Laboratory of Plant Pathology, Institut de l’Environnement et de Recherches Agricoles (INERA), Ouagadougou 01 BP 476, Burkina Faso. Laboratory of Biosciences, Université Joseph KI-ZERBO, Ouagadougou, Burkina Faso.
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Erik W. OHLSON
  • Erik W. OHLSON
  • Department of Biology, University of Virginia, Charlottesville, VA, 22904, USA.
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Michael P. TIMKO
  • Michael P. TIMKO
  • Department of Biology, University of Virginia, Charlottesville, VA, 22904, USA.
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  •  Received: 21 June 2021
  •  Accepted: 07 July 2021
  •  Published: 31 August 2021

Abstract

Brown blotch disease caused by Colletotrichum capsici, is one of the most important disease affecting cowpea production in Burkna Faso. Every year the disease can cause yield losses as high as 42 to 100% on farmer’s fileds. This study aimed to understand the inheritance of its resistance in cowpea and also identify quantitative trait loci (QTL) associated with the resistance. Seedlings of parents and F1, F2 and BC1F1 progenies from crosses involving four susceptible and four resistant parents were screened in greenhouse conditions for brown blotch disease reaction. The inheritance studies indicated that different genes action were involved in the resistance of brown blotch resistance disease. In KN-1 variety, two or several genes associated to brown blotch were suggested. In Donsin local, a partially dominant gene, Rcc1, would be associated with the resistance. In Moussa local, a single major dominant gene, Rcc2, was identified. A single major recessive gene, rcc2, was found to control resistance with brown blotch in the cowpea resistant variety IT93K-503/46-13. The research of gene or QTL associated with brown blotch in cowpea variety KN-1 has been investigated. Polymorphic single nucleotide polymorphisms (SNP) markers and QTL associated to brown blotch resistance were discovered from 63 F2 individuals from a cross involving the susceptible variety Tiligre and the resistant KN-1. More than 11800 polymorphic SNP markers covered 1019.3 cM of the whole genome of cowpea were identified. A single dominant QTL, nomed qBBDR2 and 102 individual SNP markers tightly associated with the resistance of C. capsici were identified on chromosome 2 (Phytozome) of the cowpea genome.

Key word: Cowpea, Colletotrichum capsici, resistance, inheritance, single nucleotide polymorphisms (SNP) marker, QTL.