Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 223

Full Length Research Paper

Studies on the effects of the methanol extract of Struchium sparganophora (L.) Ktze (Asteraceae) on the rat uterus

B. A. Ayinde1*, O. J. Owolabi2 and A. Okoh1
1Department of Pharmacognosy, Faculty of Pharmacy University of Benin, Benin City, Edo State, Nigeria. 2Department of Pharmacology and Toxicology, Faculty of Pharmacy University of Benin, Benin City, Edo State, Nigeria.
Email: [email protected]

  •  Accepted: 15 March 2012
  •  Published: 30 April 2012

Abstract

Struchium sparganophora (L.) Ktze (Asteraceae) is one of the vegetables equally consumed for medicinal purposes in the South Western part of Nigeria where it is used to treat a range of infections. The ethnomedical claimed use of the plant as an abortifacient crude drug was investigated on diethylstilboestrol treated rats. The methanol extract and its aqueous, and chloroform fractions were tested at the concentrations of 10 to 160 mg/ml corresponding to final bath concentrations (FBC) of 200 to 3200 µg/ml. The activities of the extract were also examined in the presence of oxytocin, acetylcholine, salbutamol and atropine. At a concentration of 10 mg/ml, the extract induced uterine contraction equivalent to 0.96 ± 0.23 g force which increased to 1.98 ± 0.07g at a concentration of 160 mg/ml. The aqueous fraction produced maximum contraction of 0.88 ± 0.33 g at the highest concentration while the chloroform fraction was inactive. While simultaneous administration of 0.008 µg/ml oxytocin or 0.004 µg/ml acetylcholine augmented the contractile effects of the extract, its activities were significantly attenuated in the presence of 1 µg salbutamol or 2 µg atropine. The results showed that the leaves of S. sparganophora have contractile effects on the uterus and partial separation of the constituents did not enhance the activities. The effects are probably mediated through the activation of the oxytocin and muscarinic receptors. 

 

Key words: Struchium sparganophora, methanol extract, uterine contraction.