Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 221

Full Length Research Paper

Immunomodulatory activities of methanol extract of the whole aerial part of Phyllantus niruri L.

Eze C. O*
  • Eze C. O*
  • Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka 410001, Enugu State, Nigeria.
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Nworu C. S
  • Nworu C. S
  • Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka 410001 Enugu State, Nigeria. 4Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.
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Esimone C. O
  • Esimone C. O
  • Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.
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Okore V. C
  • Okore V. C
  • Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka 410001, Enugu State, Nigeria.
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  •  Received: 06 December 2013
  •  Accepted: 24 April 2014
  •  Published: 31 May 2014

Abstract

In this study, the effect of methanol extract of whole aerial parts of Phyllantus niruri on some specific and non-specific immune response was investigated. The effects of P. niruri on in vivo leucocyte mobilization, delayed type hypersensitivity (DTHR) response, and humoral antibody (HA) response were determined in rats. Acute toxicity profile of P. niruri was evaluated in mice. The Agar induced in vivo leucocytes mobilization into the rats peritoneal fluid was (P<0.05) increased by P. niruri N (200 and 400 mg/kg) in a dose related manner. The total leucocytes count was higher in the extract treated group than the control group. Polymorphonuclear neutrophils (PMNS) were more mobilized than the lymphocytes. P. niruri at 100, 200 and 400 mg/kg body weight produced significant (P<0.05) inhibition of DTH response in rat by 30.55, 66.67 and 44.44%, respectively with 200 mg/kg being most significant. The primary and secondary sheep red blood cell antibody titres were significantly elevated when compared with the control group. P. niruri administered orally showed no death or signs of acute intoxication at doses up to 5000 mg/kg after 24 h of observation. The result of this study showed that the methanol extract of P. niruri whole plant possess immunomodulatory activities and warrant further investigation to determine the specific constituent(s) of the plant responsible for this property.

Key words: Phyllantus niruri, leucocyte mobilization, delayed type hypersensitivity (DTHR), humoral antibody titre, immunomodulation.