Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 223

Full Length Research Paper

The quantitation of hydroxymethylfurfural in Australian Leptospermum honeys

Sarah Windsor*, Kristyn Kavazos and Peter Brooks
Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, Queensland 4558, Australia.
Email: [email protected]

  •  Accepted: 30 November 2012
  •  Published: 31 January 2013


This research extends the rapid high-performance liquid chromatographic (HPLC) analysis of O-(2, 3, 4, 5, 6-pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA) derivatives of methylglyoxal and dihydroxyacetone in Australian Leptospermum (L.) honeys to quantify hydroxymethylfurfural (HMF) content. Results showed that among the studied honeys stored at 4°C, all six L. polygalifolium and one L. liversidgei honeys exceeded the HMF upper limit (40 mg/kg) of the International Honey Commission (IHC), while four L.liversidgei, one L. semibaccatum and one L. laevigatum honeys satisfied this IHC regulation. It was found that all of the 13 heat treated (37°C for 60 days) Leptospermumhoneys exceeded the IHC limit.


Key words: Hydroxymethylfurfural, Leptospermum, honey.