Journal of
Pharmacognosy and Phytotherapy

  • Abbreviation: J. Pharmacognosy Phytother.
  • Language: English
  • ISSN: 2141-2502
  • DOI: 10.5897/JPP
  • Start Year: 2009
  • Published Articles: 207

Full Length Research Paper

Phytochemical analysis, antioxidant and antidiarrhoeal activities of methanol extract of Fimbristylis miliacea (L.) Vahl

Ummah Hafsa Mukta
  • Ummah Hafsa Mukta
  • Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
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Roni Roy
  • Roni Roy
  • Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
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A. F. M. Shahid Ud Daula
  • A. F. M. Shahid Ud Daula
  • Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
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Mahmuda Ferdous
  • Mahmuda Ferdous
  • Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
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Anwesha Chowdhury
  • Anwesha Chowdhury
  • Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
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Sohel Mia
  • Sohel Mia
  • Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
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Afroza Akter
  • Afroza Akter
  • Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
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Israt Jahan Liya
  • Israt Jahan Liya
  • Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
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Mohammad Anwarul Basher
  • Mohammad Anwarul Basher
  • Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
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  •  Received: 24 December 2019
  •  Accepted: 21 February 2020
  •  Published: 31 March 2020

Abstract

The present study determined phytochemical content, antioxidant and antidiarrhoeal effect of methanol extract of Fimbristylis miliacea. Phytochemical screening showed the presence of flavonoids, saponins, tannins, phenols, alkaloids and cardiac glycosides. Total phenolic, flavonoids, flavonols, tannins, β-carotene, lycopene, chlorophyll-α, and chlorophyll-β contents were found 154.13 mg GAE/g, 83.14 mg QE/g, 126.45 mg QE/g, 215.72 mg GAE/g, 1.51 µg/g, 0.66 µg/g, 7.10 mg/g and 4.25 mg/g, respectively. Antioxidant potential was compared against ascorbic acid. IC50 and EC50 values were determined as indices of antioxidant potential using five experiments; IC50 in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging assay were 138.83 and 353.82 µg/ml respectively. EC50 of thiobarbituric acid assay, FRAP assay and reducing power assay were observed 2159.5, 31.59 and 129.56 µg/ml respectively. Total antioxidant capacity was 155.35 mg/g AAE. Significant reduction (p≤0.001) in fecal movement was produced by plant extract and the effect was stronger than that of the standard drug loperamide (5 mg/kg.bw). Loperamide showed 58.16% inhibition whereas extract at 400 mg/kg.bw dose inhibited 80.85% diarrhea.

Key words: Fimbristylis miliacea, phytochemical content, antioxidant, antidiarrhoeal

 

Abbreviation

DPPH, diphenyl picrylhydrazyl assay; HRSA, hydroxyl radical scavenging assay; RPA, reducing power assay; TBA, thiobarbituric acid assay; FRAP, ferric reducing antioxidant power assay; IC, inhibition concentration; EC, effective concentration; bw, body weight; min, minute; h, hour; g, gram.